This set of Protein Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Biosensors – Genetically Fused Proteins”.
1. What do you mean by GFP?
a) Gene Fluorescent Protein
b) General Fluorescent Protein
c) Green Fluorescent Protein
d) Grey Fluorescent Protein
View Answer
Explanation: GFP is the abbreviation of Green Fluorescent Protein, primarily found in jellyfish Aequorea Victoria. These proteins consist of 238 amino acids out of which 3 amino acids forms the fluorescence emitting structure. So, when they are exposed to light, the GFPs emit green fluorescence.
2. The molecular weight of GFP is 27 kDa.
a) True
b) False
View Answer
Explanation: The given statement is true. Green Fluorescent Proteins has a molecular weight of 27 kDa and possess a structure resembling a cylinder. These proteins are employed in the development of genetically encoded fluorescent biosensors.
3. What is the full form of FRET?
a) Fluorescence Radioactivity Energy Transfer
b) Fluorescence Resonance Energy Transfer
c) Fluorescence Radioactivity Energy Tuning
d) Fluorescence Resonance Energy Tuning
View Answer
Explanation: The full form of FRET is Fluorescence Resonance Energy Transfer. It acts as an important tool for genetically engineered subjects that report on the conformation state of proteins by transferring energy from donor to the acceptor.
4. Which of the following biosensor is not used in both food industries as well as in drug testing procedures?
a) Bacterial biosensors
b) DNA biosensors
c) Enzyme biosensors
d) Immune biosensors
View Answer
Explanation: DNA biosensors are used for the purpose of diagnosing diseases and genetic monitoring. For drug testing, enzyme biosensors, immune biosensors and cell-based sensors are used whereas in food industries, bacterial biosensors are predominantly used.
5. Who is the father of biosensors?
a) J. Presper Eckert Jr
b) Leland C. Clark Jr
c) Alan Mathison Turing
d) Janusz Bryzek
View Answer
Explanation: The first real biosensor was developed by Leland C. Clark Jr in 1956 for determining the amount of glucose in blood. Hence, he is known as the father of biosensors. But the biosensors were commercially produced originally in the year 1975.
6. What do you mean by the term bioreceptor?
a) It is the molecule that transduce signals
b) It is used to quantify the signal that has been transduced
c) It is a molecule that specifically recognizes an analyte
d) It is an element that converts one form of energy into another
View Answer
Explanation: Bioreceptor is a molecule that precisely recognizes an analyte. Examples of bioreceptors include antibodies, DNA molecules, enzymes etc. The process of generating signals upon interaction of the bioreceptor with an analyte is termed as bio-recognition.
7. Which of the following statement is correct regarding FRET?
a) It allows to study the dynamics of signaling molecular activities in a living cell
b) It is a radioactive phenomenon of energy transfer
c) It has low signal-to-noise ratio
d) It triggers fluorescence emission of the donor
View Answer
Explanation: FRET allows us to study the dynamics of signaling molecular activities in living cells. It is a non-radioactive phenomenon of energy transfer, in which the energy emitted by the donor triggers fluorescence emission of the acceptor and it has a relatively high signal-to-noise ratio.
8. Which of the following is the most commonly used electrochemical biosensors?
a) Graphene-based biosensors
b) Quartz crystal biosensors
c) Magnetoelectric biosensors
d) Pedometric biosensors
View Answer
Explanation: Graphene-based biosensors are the most commonly used electrochemical biosensors. It measures the electrical signals produced during a chemical reaction that occurs between the target molecule and the recognition element.
9. The sensing device in the genetic biosensors consists of which of the following?
a) DNA aptamer
b) Post-translational activator and Post-translational repressor
c) Translational activator and Translational repressor
d) Transcriptional activator and Transcriptional repressor
View Answer
Explanation: The sensing device consists of transcriptional activator and transcriptional repressor, which is comprised of a DNA-binding domain and a ligand-binding domain or a riboswitch containing an RNA aptamer.
10. TF-based genetic biosensors is a prevailing tool for real-time monitoring of natural products.
a) True
b) False
View Answer
Explanation: TF-based genetic biosensors are not only employed in synthesizing natural products but also acts as an important tool for real-time monitoring. Similarly, they are involved in detecting intermediates or end products in a biological pathway.
Sanfoundry Global Education & Learning Series – Protein Engineering.
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