Basic Biotechnology Questions and Answers – Technology of Mammalian and Plant Cell Culture

This set of Basic Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Technology of Mammalian and Plant Cell Culture”.

1. In fluidized bed fermenter the estimated value of µmf/ µt is __________
a) 0.05-0.07
b) 0.02-0.1
c) 0.001-0.002
d) 0.003-0.004
View Answer

Answer: b
Explanation: In fluidized bed fermenter the estimated value of µmf/ µt is 0.02-0.1. µt is terminal velocity. Substrate pellet is sin air.

2. Which one does continuous sterilization does not include?
a) Heating period
b) Holding period
c) Cooling period
d) Wash period
View Answer

Answer: d
Explanation: Batch sterilization includes heating and holding period. It does not have cooling period. But continuous sterilization includes all the three.

3. Which of the following is used to introduce genes into the dicot plants?
a) Microinjection
b) Electrophoresis
c) Ti-plasmid infection
d) SDS Page
View Answer

Answer: c
Explanation: Ti plasmid infection is tumor inducing plasmid infection. Agrobacterium tumefaciens are commonly used for this process. Electrophoresis is to separate charged biological compounds on the basis of molecular weight.

4. Which technique is best for insecticide resistant plant?
a) Ouchterlony method
b) Mutant selection
c) Allele selection
d) Gene fusion
View Answer

Answer: b
Explanation: Insecticides are toxins used to kill the pests. Sometimes it can harm the crop plant too. Mutant selection method best selects insecticide resistant plant.

5. Which cell based technology combines two cells without cell wall?
a) Protoplast fusion
b) Somatic mutation
c) Clonal mutation
d) Gene fusion
View Answer

Answer: a
Explanation: Somatic mutation is a kind of genetic aberration. Protoplast fusion technology is used to fuse two cells. It does not involve cell wall fusion.

6. How is delayed ripening achieved?
a) Gene silencing
b) Protoplast fusion
c) Monoclonal antibody
d) Antisera injection
View Answer

Answer: a
Explanation: RNA interference is used to achieve this. It involves introduction of a complementary RNA to stop protein translation. It is also called gene silencing.

7. Which genetically altered organism was the first field test conducted on?
a) Strawberry seedlings
b) Bt cotton
c) Tomato
d) Pomato
View Answer

Answer: a
Explanation: Pomato is a hybrid of potato and tomato. Bt cotton is a transgenic plant. First field test for a genetically altered organism was in case of strawberry seedlings.

8. What does transplastomics target?
a) Genes in cytoplasmic pool
b) Genes in chloroplast
c) Genes in mitochondrion
d) No genes
View Answer

Answer: b
Explanation: As the name suggest transplatomics target genes in plastid. Plastids having genes are chloroplast. The genes in chloroplast are targeted.

9. What is something that can be achieved by plant breeders but not by animal breeders?
a) Clonal propagation
b) Sample loading
c) Sample transfer
d) Sample analysis
View Answer

Answer: a
Explanation: Plant breeders can easily achieve clonal clonal propagation. This cannot be done for animal cells. This way plant breeders are at advantage than the animal breeders.

10. What is the elaboration of ORI?
a) Origin of replicon
b) Origin of replication
c) Origins of replicating
d) Original replication
View Answer

Answer: b
Explanation: ORI is a gene sequence. It stands for origin of replication. Replication starts only if ORI is present.

11. Which among the following is an important component necessary for Ti-plasmid integration in plant genome?
a) Tumor inducing gene
b) Transmembrane sequences
c) B cells
d) T cells
View Answer

Answer: a
Explanation: Ti gene is an important component of Ti-plasmid. It is tumor inducing gene. It is generally found in Agrobacterium.

12. Which cell based technology gives cell with ability to harness more energy?
a) Monochromatic absorption
b) Chlybridisation
c) Chemisorption
d) Physisorption
View Answer

Answer: b
Explanation: Chlybridisation is used to harness more energy in cell. It is a cell based technology. Sorption are a kind of adsorption.

Sanfoundry Global Education & Learning Series – Basic Biotechnology.

To practice all areas of Basic Biotechnology, here is complete set of Multiple Choice Questions and Answers.

If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]

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Manish Bhojasia - Founder & CTO at Sanfoundry
Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

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