This set of Plant Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Protoplast Culture”.
1. Who coined the term protoplast?
a) Stephen Hales
b) Thomas Nuttall
c) Johannes von Hanstein
d) James Bolton
View Answer
Explanation: The term protoplast was first coined by Johannes von Hanstein in the year 1880. This term was used to describe an entire cell excluding the cell wall. After in the year of 1892, Von Klercker carried out the first protoplast isolation.
2. Protoplasts are isolated only by using enzymatic method.
a) True
b) False
View Answer
Explanation: The given statement is incorrect. The Protoplasts are isolated either by using enzymatic method or mechanical method. Comparatively, the mechanical method yields poor protoplast hybrids. hence, enzymatic method is often preferred.
3. What do you mean by plasmolyticum?
a) Solution that inhibits plasmolysis
b) Solution that causes plasmolysis
c) Medium containing protoplasts
d) Protoplast hybrids resulted from somatic fusion
View Answer
Explanation: Plasmolyticum is a solution or a substance that causes plasmolysis. Usually, in mechanical method, the cells are kept in a suitable plasmolyticum in order to release out the protoplast after cutting the cell with a fine knife.
4. What is CPW in protoplast culturing technique?
a) Cytosolic protoplast wastes
b) Cytosolic protoplast washing
c) Cell and protoplast wastes
d) Cell and protoplast washing
View Answer
Explanation: CPW means cell and protoplast washing. They are available in salt concentrations and are also used for dissolution of enzymes. CPW salt solutions are generally added to the protoplast suspension and centrifuged for 5 minutes. And they typically contain 13% mannitol.
5. Which of the following term is used to denote isolated protoplasts?
a) Naked cells
b) Derived cells
c) Cell mass
d) Distinguished cells
View Answer
Explanation: The isolated protoplasts are also called as naked cells because during isolation process, the cell walls are removed and this causes the plasma membrane to be completely exposed to the outer environment.
6. Which of the following is an advantage of enzymatic method of protoplast isolation?
a) Mostly used for vacuolated cells
b) Osmotic shrinkage is maximum
c) Cells remain intact
d) Yield and viability of are unpredictable
View Answer
Explanation: The advantages of enzymatic method of protoplast isolation include high yield and viability when compared to mechanical method; this method is used for variety of plant cells; osmotic shrinkage in enzymatic method is low and most crucially the cells remain intact and less damaged.
7. Which of the following is a fusogenic agent used in protoplast culture?
a) PEG
b) PVC
c) Salicylic acid
d) Auxin
View Answer
Explanation: The most commonly used fusogenic agent in protoplast culturing technique is polyethylene glycol (PEG). It is used to fuse isolated protoplasts which will result in somatic hybrids. Other fusogenic agents include polyvinyl alcohol, sendai virus etc.
8. Cell density is one of the major factors influencing protoplast cultures.
a) True
b) False
View Answer
Explanation: The given statement is true. Cell density is one of the major factors that influence protoplast cultures. It affects plating efficiency and even influence the survival rate of protoplasts in the culture medium.
9. Which of the following is not a method used to culture protoplast?
a) Hanging drop culture method
b) Using micro culture chambers
c) Using soft agar matrix
d) Dendritic culture method
View Answer
Explanation: Different methods are employed for culturing protoplasts. They are Hanging drop culture method, culturing using micro culture chambers and also soft agar matrix method. Dendritic cell cultures are used for producing antigen presenting cells.
10. What is the optimum pH for protoplast culture?
a) 4.5 to 4.9
b) 5.5 to 5.9
c) 6.5 to 6.9
d) 7.5 to 7.9
View Answer
Explanation: The optimum pH range for protoplast culture is 5.5 to 5.9. At this pH values, the isolated protoplasts are cultured and the cell walls are reformed to generate callus. And it is also necessary to maintain the auxin to cytokinin ratio for obtaining good somatic hybrids.
Sanfoundry Global Education & Learning Series – Plant Biotechnology.
To practice all areas of Plant Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.
If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]
