This set of Basic Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Media Design for Fermentation Processes”.
1. Which among the following is not a major part of biotechnological program?
a) Selection
b) Screening
c) Culture
d) Petrochemical
View Answer
Explanation: Selection is an important activity of biotechnological program. Screening also exhibits the same. Screening is the use of procedure to allow detection and isolation.
2. What needs to be preserved with minimum degeneration of genetic properties?
a) Consumer
b) Producer microbes
c) E. coli
d) Streptococci
View Answer
Explanation: Producer microbes require to be preserved with minimum degeneration of genetic qualities. These are generally preserved on agar medium. It is done by reduced metabolism.
3. How can genomes be modified?
a) Alliteration
b) Gene shot
c) Mutagenesis
d) Genetic lysis
View Answer
Explanation: Genomes can me modified by mutagenesis. This can also be achieved my various types of hybridization. Mutational programs are aimed at strain improvement.
4. Which among the following is not a mutagen?
a) Ultraviolet radiation
b) Ionizing radiation
c) Chemical mutagens
d) Nuclease
View Answer
Explanation: Mutagens available includes ultraviolet and ionizing radiations. Chemical mutagens are also available. Mutagens programs are primarily aimed at strain improvement.
5. What includes procedures to allow detection and isolation of microbes of interest?
a) Mashing
b) Screening
c) Precipitation
d) Centrifuging
View Answer
Explanation: Screening is the use of procedures to allow detection and isolation. It is for microbes of interest among high population. Metabolites can also be taken.
6. Which among the following is not a method to preserve producer microbes?
a) Reduced metabolism
b) Drying
c) Freeze drying
d) Sonography
View Answer
Explanation: Producer microbes are preserved using special techniques. It includes reduced metabolism, drying, freeze drying. Ultra-low temperature devices can also be used.
7. What are mutational programs aimed at?
a) Culture production
b) Genomic lysis
c) Strain improvement
d) Incubation
View Answer
Explanation: Mutational programs are aimed at strain improvement. Genomes can be modified. Mutagenesis or hybridization is used.
8. How does impeller speed affect bubble size?
a) As speed increases, bubble size increases
b) As speed increases, bubble size is constant
c) As speed increases, size becomes inconsistent
d) As speed increases, size decreases
View Answer
Explanation: As the impeller speed increases, shear also increase. With shear, turbulence increases. This decrease the bubble size.
9. Foaming may be caused by ________
a) Agitation decreases
b) Large stable bubbles formed
c) Breaking down of large unstable bubbles
d) Small unstable bubbles formed
View Answer
Explanation: Large weak foams are formed. It can be easily broken. Agitation forms new small and unstable bubbles.
10. What is the net yield of ATP and NADH per mole of glucose?
a) 2 ATP and 0 NADH
b) 1 ATP and 1 NADH
c) 3 ATP and 2 NADH
d) 2 ATP and 1 NADH
View Answer
Explanation: Lactic acid fermentation is a biological process. Sugars are converted to energy. 2 ATP and 0 NADH are produced per mole of glucose.
11. In a chemostat operating under steady state, bacterial culture can be grown at dilution rate higher maximum growth rate by _______
a) partial cell recycling
b) sub optimal temperature
c) pH cycling
d) substrate feed rate cycling
View Answer
Explanation: Biomass can be cultured by partial cell recycling. It is removed from system. Cell concentration progressively decreases.
12. Which approach is correct to cultivate microbes under physiological conditions?
a) Fed batch
b) Continuous
c) Batch
d) Discontinuous
View Answer
Explanation: Data from continuous culture is much less complex. There is dynamic equilibrium. Small excursions from steady state is seen.
Sanfoundry Global Education & Learning Series – Basic Biotechnology.
To practice all areas of Basic Biotechnology, here is complete set of Multiple Choice Questions and Answers.
