This set of Basic Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Solid Substrate Fermentation”.
1. Dilution rate and limiting a specific nutrient is an important parameter for _________
a) Fed batch
b) Batch
c) Chemostat
d) Turbidostat
View Answer
Explanation: Low number of cells are present in bioreactor. Cells can grow at higher growth rates. Growth is not limited by addition of nutrient.
2. Aeration in a bioreactor is provided by ______
a) Impeller
b) Baffles
c) Sparger
d) Temperature set
View Answer
Explanation: Bioreactor has aeration mechanisms. Sparger does aeration in bioreactor. Temperature set is to control temperature.
3. Baffles are incorporated in bioreactor to _______
a) Prevent vortexing
b) Maintain uniform suspension of cells
c) Minimize the size of air bubble
d) Maintain uniform medium
View Answer
Explanation: Baffles are incorporated in bioreactor. It is to prevent the vortexing. It is to improve aeration efficiency.
4. A sample collected shows doubling of cell number per unit time. The culture is in the _______
a) Lag phase
b) Log phase
c) Stationary phase
d) Death phase
View Answer
Explanation: Lag, log, stationary and death phases are in microbial growth curve. Doubling of cell number per unit time shows lag phase. In stationary phase, there is no production.
5. In growth curve graph of biomass against time, slope represents __________
a) Specific growth rate
b) Specific death rate
c) Substrate concentration
d) Product formation
View Answer
Explanation: Growth curve graphs are plotted for studies. As such biomass is plotted against time. The slope represents specific growth rate.
6. What is the preferred method of sterilization in large scale fermentation?
a) Chemicals
b) Radiation
c) Filtration
d) Heat
View Answer
Explanation: Large scale fermentation processes are in demand in recent industry. Various methods of sterilization are used. Heat method is the commonly used one.
7. In case more than two carbon source is present in medium, how many log phases appear?
a) 3
b) 1
c) 2
d) 4
View Answer
Explanation: Sometimes more than one carbon source might be present in medium. Suppose glucose and sucrose are added in growth medium. 2 log phases can be seen.
8. The death rate can be termed as ______
a) Exponential
b) Constant
c) Cannot be predicted
d) Non-exponential
View Answer
Explanation: Death phase is one of the phase in microbial growth curve. It is an exponential phase. Number of calls have a steady decrease.
9. Consider a continuous culture. Steady state is dependent on ______
a) Flow rate
b) Dilution rate
c) Specific growth rate
d) Sigmoid curve
View Answer
Explanation: Steady state is dependent on dilution rate. There are various culture techniques. These include batch, fed batch and continuous.
10. Solid state fermentation is best suited for __________
a) Filamentous fungi
b) Yeast
c) Bacteria
d) Slimes
View Answer
Explanation: Solid state fermentation is not so suitable for unicellular organisms. It is best suited for multicellular fungi. Filamentous fungi best exhibit the same.
Sanfoundry Global Education & Learning Series – Basic Biotechnology.
To practice all areas of Basic Biotechnology, here is complete set of Multiple Choice Questions and Answers.
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