This set of Basic Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Bioprocess Technology – The Bioreactor”.
1. Environmental parameters include temperature, pH and aeration in biotechnological processes.
a) True
b) False
View Answer
Explanation: Environmental parameters include temperature, pH and aeration in biotechnological processes. It is optimally maintained in process control. These help in the growth of organism.
2. E. coli is mostly prevalent in the intestinal tracts of warm-blooded and cold-blooded animals.
a) True
b) False
View Answer
Explanation: Escherichia coli is a bacterium used as a cloning vector. It is mostly prevalent in the intestinal tracts of warm-blooded and cold-blooded animals.
3. Which among the following are not secondary metabolites?
a) Amino acids
b) Antibiotics and alkaloids
c) Naphthalene and peptides
d) Growth factors
View Answer
Explanation: Secondary metabolites have important ecological functions. Primary metabolites are involved in direct growth. Amino acids are primary metabolites.
4. What does the term Ks mean, in the following equation?
μ = μmax\(\frac{S}{Ks+S}\)
a) Specific growth
b) Concentration
c) Maximum specific growth rate
d) Saturation constant
View Answer
Explanation: This equation is by Monad. S is concentration of substrate. Ks is saturation constant.
5. Which among the following is not a way for growing microbes in bioreactor?
a) Batch
b) Fed-batch
c) Continuous
d) Potent
View Answer
Explanation: There are three main ways of growing microbes in bioreactor. These are batch, fed-batch and continuous. Cultures can be grown in the presence or absence of oxygen.
6. How can microbes be attached to surfaces?
a) Immobilization
b) Seed plate
c) Shake flask
d) Orbital shaker
View Answer
Explanation: The microbes can be free. They can also be attached to surfaces. It can be done by immobilization or natural adherence.
7. Fixed volume of microbes are taken in which culture?
a) Batch culture
b) Seed culture
c) Log culture
d) Med culture
View Answer
Explanation: Microbes are inoculated in fixed volume. This happens in case of batch culture. Growth takes place and products of growth accumulate.
8. Which among the following is not a growth phase?
a) Lag phase
b) Log phase
c) Stationary phase
d) Shoot phase
View Answer
Explanation: The initial phase is lag phase. The transient acceleration phase is exponential phase. Growth rate decreases in deceleration phase.
9. What are the containment vehicles of any biotechnological production process?
a) Cooker
b) Bioreactor
c) Plate
d) Autoclave
View Answer
Explanation: Bioreactors are the containment vehicles of any biotechnological production process. Process includes brewing, organics or amino acids. Enzymes and antibiotics also used these.
10. In what phase are the microbes suspended in nutrient medium?
a) Gas
b) Aqueous
c) Solid
d) Rigid
View Answer
Explanation: Inside the bioreactor, the microbes are suspended in aqueous nutrient medium. It contains necessary substrates for growth. It is required for product formation.
11. In which culture microbes are inoculated in a fixed volume of medium?
a) Batch
b) Console
c) Retrieve
d) Cellar
View Answer
Explanation: In batch culture microbes are grown n fixed volume of medium. Along with growth, nutrients are consumed. The environment within the bioreactor is continuously changing.
12. Which phase is a time of no apparent growth?
a) Lag
b) Deterioration
c) Exponential
d) Log
View Answer
Explanation: Lag phase is the phase of no apparent growth. Cells are in the process of adapting to the new environment. New growth eventually begins.
Sanfoundry Global Education & Learning Series – Basic Biotechnology.
To practice all areas of Basic Biotechnology, here is complete set of Multiple Choice Questions and Answers.
