This set of Basic Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Genetics – Nucleic Acid Probes”.
1. Heating of the double stranded DNA to separate into two complementary strands is called as ____
a) Denaturation
b) Splicing
c) Polymerization
d) Ligation
View Answer
Explanation: Denaturation is the process that involves a double stranded DNA. It is heated at around 95-98 degree Celsius. This is done to split the DNA into 2 complementary single strands.
2. What are designed to detect specific target DNA molecules?
a) Ribose sugar probes
b) Nucleic acid probes
c) Phosphodiester bond probes
d) Ammonia probes
View Answer
Explanation: Probes are generally used to detect specific targets. In molecular biology also, probes are used. Nucleic acid probes are designed to detect specific target DNA molecules.
3. Nucleic acid hybridization depends on the ability of _________
a) Probe
b) Golgi apparatus
c) Centriole
d) Vacuole
View Answer
Explanation: Nucleic acid hybridization depends on the ability of probe. It is single stranded probe nucleic acid. It must become attached or annealed to complementary single strand.
4. Nucleic acid probe must get attached to __________
a) Ribosome
b) Complementary single strand
c) Centriole
d) Protein
View Answer
Explanation: Nucleic acid probe must get attached to complementary single strand. This is also called annealing. Target sequences are single stranded.
5. Apart from the complementary single stranded target, the population also has _________
a) Non-complementary nucleic acid molecules
b) Non-complementary proteins
c) Non-complementary mitochondrion
d) Non-complementary telomere
View Answer
Explanation: The gene population has the complementary single stranded target. It also has Non-complementary nucleic acid molecules. This forms the total sequence.
6. Single stranded target sequences are complementary to the nucleic acid probe.
a) True
b) False
View Answer
Explanation: Single stranded target sequences are complementary to the nucleic acid probe. Nucleic acid probe must get attached to complementary single strand. This process is also termed as annealing.
7. Test samples must be treated so that the cells are ___________
a) Lysed
b) Swollen
c) Shrink
d) Grow
View Answer
Explanation: Test sample consists of the required gene sequences. These are packed of in the cells. So, the test sample must be treated so the cells are lysed and products come out.
8. Double stranded DNA is denatured into _____________
a) Single stranded forms
b) Complementary proteins
c) RNA
d) mRNA
View Answer
Explanation: The cells are lysed and product is out. The DNA is denatured. It then turns into single stranded forms.
9. The objective of the probe is to be ___________
a) Readily detected
b) Readily diluted
c) Synthesized
d) Lysed
View Answer
Explanation: The probe is sent so that it is readily detected. For this radionucleotides are incorporated in. Otherwise fluorescent compounds can be used in place of radionucleotides.
10. Probe-based technical systems are obtained by ____________
a) Immobilizing mitochondrion
b) Immobilizing bacteria
c) Immobilizing virus
d) Immobilizing probes
View Answer
Explanation: Probe-based technical systems are obtained by Immobilizing probes. Single stranded target sequences are complementary to the nucleic acid probe. Nucleic acid probe must get attached to complementary single strand.
11. Immobilizing the probe and easily washing of the unhybridized DNA is called as ______________
a) Solid phase hybridization
b) Two on two hybridization
c) S on s hybridization
d) C on c hybridization
View Answer
Explanation: Probes are immobilized. So unhybridized DNA is easily washed off. This is called as solid phase hybridization.
12. Microarrays are intrinsically miniaturized extensions of conventional tests of ________
a) Nucleic acid hybridization
b) Protein hybridization
c) Mitochondrial hybridization
d) Nuclear hybridization
View Answer
Explanation: Microarrays are intrinsically miniaturized extensions of nucleic acid hybridization. Microarrays are also called DNA chips. These are solid supports of DNA.
Sanfoundry Global Education & Learning Series – Basic Biotechnology.
To practice all areas of Basic Biotechnology, here is complete set of Multiple Choice Questions and Answers.
