This set of Plant Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Culture Environment & Media”.
1. Who is the father of plant tissue culture?
a) William Roxburgh
b) Antoine Lavoisier
c) Gottlieb Haberlandt
d) Robert Brown
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Explanation: The father of plant tissue culture is Gottlieb Haberlandt, who also discovered the phenomenon of totipotency. He inferred through his findings that plant cells are capable of developing into a whole organism.
2. Cultures are incubated only in dark condition.
a) True
b) False
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Explanation: The given statement is incorrect. Tissue cultures are incubated in both light and dark conditions based on their activity. Usually, the cultures are incubated at 26 ± 2°C, after which they are maintained under photoperiod settings for 16 hours and at darkness for 8 hours.
3. What does explant in plant tissue culture mean?
a) The removed plant tissues that are cultured in the medium
b) The plant from which the tissue is isolated
c) The culture medium used
d) The lateral portion of a plant
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Explanation: Explant is the tissue which is removed from a plant in order to be cultured in the nutrient medium under aseptic sterile conditions. Explants are obtained from various plant parts and they are used to produce large number of vegetative plantlets in invitro atmosphere.
4. What is the optimum pH for most plant tissue cultures?
a) 4.1 to 4.7
b) 5.2 to 5.8
c) 6.3 to 7.4
d) 7.6 to 8.5
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Explanation: Most plant tissue cultures grow between the pH range of 5.2 to 5.8. In some cases, plant tissue cultures exhibit growth even around 7.5 pH but, above which, the culture medium becomes unsuitable for tissue growth.
5. Which is the most commonly used culture medium?
a) B4 medium
b) B5 medium
c) MS medium
d) W medium
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Explanation: The most commonly used culture medium is the Murashige and Skoog medium or MS medium. It was developed by two scientists named, Toshio Murashige and Folke K. Skoog in the year 1962, while discovering plant growth regulators.
6. Nitsch medium is also called as B5 medium.
a) True
b) False
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Explanation: The given statement is incorrect. The term B5 is used to denote Gamborg medium. The B5 medium was developed by O. L. Gamborg in the year 1968. This medium is specifically used for protoplast culture technique.
7. Which of the following is an amino acid used in plant tissue culture?
a) Nicotinic Acid
b) Casein hydrolysate
c) Riboflavin
d) Myoinositol
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Explanation: Amino acids are used as a source of nitrogen as they can be easily assimilated by plants, when compared to inorganic forms of nitrogen. One of the most frequently used amino acid constituent is the Casein hydrolysate.
8. The only solidifying agent used for culture medium is agar.
a) True
b) False
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Explanation: The given statement is incorrect. In general, two types of culture media are used. They are agar and gelatin. Agar is known as the universal gelling agent and its concentration ranges between 0.8 to 1 %. And gelatin is used in cases where the culturing temperature is lower than 25°C.
9. Which medium was the first to be developed for root culture?
a) B4 medium
b) B5 medium
c) MS medium
d) W medium
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Explanation: The medium which was first developed for root culture is the W medium or White medium. This medium was first designed by P. R. White in the year 1963 for the purpose of establishing the root culture for tomato.
Sanfoundry Global Education & Learning Series – Plant Biotechnology.
To practice all areas of Plant Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.
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