This set of Plant Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Cell Suspension Culture”.
1. Who discovered cell culture?
a) Karl Ernst von Baer
b) Panchanan Maheswari
c) Ross Granville Harrison
d) Charles Bonnet
View Answer
Explanation: The cell culture was first discovered by an American embryologist named, Ross Granville Harrison during early twentieth century. He isolated the culture from the embryonic tissue of a frog and grew them in vitro.
2. All cell suspension cultures are continuous cultures.
a) True
b) False
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Explanation: The given statement is incorrect. Cell suspension cultures are either continuous or batch. In continuous cultures, nutrient depletion does not occur due to the continuous flow. In batch culture, in order to avoid nutrient depletion, it is necessary to maintain the culture by subculturing technique.
3. Which of the following is not a type of batch cell suspension culture?
a) Slow rotating culture
b) Fast rotating culture
c) Shaker culture
d) Spinning culture
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Explanation: Different types of batch cell suspension cultures include, slow rotating culture, shaker culture, spinning culture and stirred culture. Of all the four types, shaker culture is the most effective batch culture technique.
4. Which of the following is a disadvantage of continuous cell suspension culture?
a) Nutrients cannot be adjusted
b) This system does not support large scale production
c) Plant regeneration is impossible
d) Slower growth and lower productivity
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Explanation: Continuous cell suspension cultures are suitable for large scale production and the nutrient supply can be adjusted continuously. It is apparent that whole plant can be regenerated from a single cell. The major drawback of continuous cell suspension culture is slower growth and lower productivity.
5. Turbidostat is a continuous culturing method.
a) True
b) False
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Explanation: The given statement is correct. Turbidostat is a continuous culturing method, which is much similar to a chemostat. In turbidostat, the turbidity is held constant throughout the process by changing the feed rate.
6. What is the usual speed of magnetic stirrer in stirred culture?
a) 50 to 100 rpm
b) 100 to 200 rpm
c) 200 to 600 rpm
d) 600 to 1000 rpm
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Explanation: In a stirred batch culture system, the usual speed of magnetic stirrer is around 200 to 600 rpm. And this technique is mainly employed for large scale production. Magnetic stirrers are used to agitate the culture safely and effectively.
7. Which of the following statement is incorrect regarding cell suspension cultures?
a) Cell suspension cultures are used to obtain multiple cell clones
b) They are used for biochemical studies
c) They are used to understand metabolic pathways
d) Cell suspension cultures are suitable for large scale productions
View Answer
Explanation: Cell suspension cultures are used to obtain single cell clones. Single cell clones have a variety of applications in morphological as well as biochemical studies. Cell suspension cultures are also used to understand metabolic pathways.
Sanfoundry Global Education & Learning Series – Plant Biotechnology.
To practice all areas of Plant Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.
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