This set of Basic Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Potential Laboratory Biohazards of Genetic Engineering”.
1. Increase in what can predict the growth of cell?
a) Cell material
b) Replication
c) Translation
d) Transcription
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Explanation: Growth of organism can be seen as the increase of cell material. It is expressed in terms of mass or cell number. It results from enzymatically catalyzed biological steps.
2. The enzyme that is used in PCR is _______
a) RNA Polymerase
b) Taq polymerase
c) Ribonuclease
d) Endonuclease
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Explanation: PCR is polymerase chain reaction. Taq polymerase is a thermostable DNA polymerase. It is used in PCR.
3. Taq polymerase is named after _____
a) Haemophilus influenzae
b) Thermo aquifaciens
c) Thermus aquaticus
d) Salmonella typhi
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Explanation: Taq polymerase is named after Thermus aquaticus. It is frequently used in PCR. It is a method for greatly amplifying short DNA fragments.
4. Where is systems biology not used?
a) Ecology
b) Immunology
c) Developmental biology
d) Automation
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Explanation: Systems biology endeavors to interpret complex biological systems. It integrates all level of functional information. It is not used in automation.
5. What does the construction of recombinant DNA molecules and their insertion into microbes create?
a) Novel organisms
b) Viruses
c) Viroid
d) Lichen
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Explanation: The construction of recombinant DNA molecules and their insertion into microbes create novel organisms. These might be released from laboratory. It can become a biohazard to humans or environment.
6. Newly synthesized organisms might face difficulties to sustain in environment.
a) True
b) False
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Explanation: Newly synthesized organisms would not be able to compete with normal strains. Normal strains are present in nature. These were the views of genetic manipulation once.
7. The standards of containment enforced in the early years of recombinant DNA studies were unnecessarily restrictive.
a) True
b) False
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Explanation: Experiments involve physical and biological containment of organism. The standards of containment enforced in the early years of recombinant DNA studies were unnecessarily restrictive. There has been a relaxation in regulations recently.
8. Standards remain stringent for the study of ________
a) Pathogenic microbes
b) Fishes
c) Agnatha
d) Jellyfish
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Explanation: Standards remain stringent for the studies of pathogenic organism. Because they might cause harmful effects. There is no relaxation in regulations.
9. Which among the following is not needed for pathogenic studies?
a) Negative-pressure laboratories
b) Autoclaves
c) Safety cabinet
d) Lathe
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Explanation: Highly skilled personnel are needed for pathogenic studies. Correct physical containment equipment are needed. Some examples of such are negative-pressure laboratories, autoclaves and safety cabinet.
10. What can be achieved by selecting non-pathogenic strains?
a) Biological containment
b) DNA
c) RNA
d) Ribosome
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Explanation: Biological containment can be achieved by selecting non-pathogenic strains. These strains are for cloning. These are deliberate genetic manipulation of organism.
11. A most widely used cloning agent is __________
a) E. coli
b) Homo sapiens
c) Vitis vinifera
d) Ascaris
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Explanation: E. coli is Escherichia coli. It is a bacterium. It is used as a cloning vector.
12. What is the optimum temperature for activity of Taq polymerase?
a) 75-80 °C
b) 85-90 °C
c) 65-70 °C
d) 50-55 °C
View Answer
Explanation: Taq’s optimum temperature for activity is 75-80 °C. It has a half-life of greater then 2 hours at 92.5°C. It is able to withstand protein denaturing conditions.
Sanfoundry Global Education & Learning Series – Basic Biotechnology.
To practice all areas of Basic Biotechnology, here is complete set of Multiple Choice Questions and Answers.
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