This set of Marine Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Aquaculture and Mariculture”.
1. _______________ is the first behavioral sign of a fish disease.
a) Reduced feeding
b) Reduced breeding
c) Increased swimming pattern
d) Loss of scales
View Answer
Explanation: In both fish and shell fish the first indication of disease is when the organism stops feeding. In moderate to severe cases, fish can be seen in moribund position in nooks and corner in the water body.
2. Which is a fish model used for biomedical research of human diseases?
a) Denison barb
b) Pearl oyster
c) Goldfish
d) Medaka
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Explanation: Medaka or Oryzias latipes is a fish model used extensively for biomedical research. In comparison to Zebrafish (Danio rerio) the Medaka is less popular and often used together with the former for modeling of human diseases.
3. Hemotoxylin is a nuclear dye.
a) True
b) False
View Answer
Explanation: Hemotoxylin is a nuclear dye that stains the negatively charged DNA while eosin stains the positively charged organelles in the cytoplasm. Both these dyes are used in histology examination of diseased fish tissues.
4. 40:1 is the appropriate fixative ratio used in tissue fixative for histology.
a) True
b) False
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Explanation: Fixatives such as formalin are used to fix the tissue for histology analysis in order to analyze the fish pathology. In the ratio of 40:1 the fixative is used to inhibit the activity of naturally occurring enzymes that may degrade the tissue.
5. Which is a method to detect presence of active virus particles in the tissue?
a) Electron microscopy
b) FISH
c) PCR
d) Chromatography
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Explanation: Many viral pathogens such as the White Spot Syndrome Virus pose major risk to aquaculture, causing mortality within a few days of infection. Electron microscopy (SEM and TEM) is a method to detect the presence of active virions in a tissue.
6. WSSV (White Spot Syndrome Virus) is a ________________ virus.
a) ssRNA
b) dsRNA
c) ssDNA
d) dsDNA
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Explanation: The WSSV is a double-stranded circular DNA virus with a genome size ranging from 281-312 kbp. It targets the ectoderm and mesoderm tissues of culture shrimps causing pigmentation and white spots.
7. The Yellow-head virus replicates in _________________
a) golgi apparatus
b) mitochondria
c) nucleus
d) cytoplasm
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Explanation: The Yellow-head virus is the second most serious viral pathogen after WSSV that infects the aquaculture species of penaeid shrimps. It replicates in the cytoplasm and causes systemic necrosis of ectodermal and mesodermal tissues.
8. Which is not a sign of Infection myonecrosis virus pathology?
a) White spots in uropods
b) Opaqueness of abdomen
c) Necrosis of skeletal muscles
d) Inclusion bodies
View Answer
Explanation: White spots in the carapace of penaeid shrimps are caused by the WSSV, while other signs mentioned above are observed during infectious myonecrosis viral pathology. It is a double-stranded RNA.
9. Which is true for pathology caused by Taura Syndrome Virus?
a) No survivors result’
b) Survivors get rid of virus
c) Survivors carry virus
d) Survivors can not transmit the virus
View Answer
Explanation: Taura syndrome virus is a linear single-stranded RNA virus that causes red coloration and necrosis in the cuticular epithelium. Survivors of the disease carry the virus for life and can transmit it vertically.
More MCQs on Aquaculture and Mariculture:
- Aquaculture and Mariculture MCQ (Set 2)
- Aquaculture and Mariculture MCQ (Set 3)
- Aquaculture and Mariculture MCQ (Set 4)
Sanfoundry Global Education & Learning Series – Marine Biotechnology.
To practice all areas of Marine Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.
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