This set of Agricultural Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Genetic Engineering Enzymes – Reverse Transcriptase”.
1. Reverse transcription should be carried out with a maximum amount of mRNA.
a) True
b) False
View Answer
Explanation: Reverse transcription should be carried out with a defined minimum amount of mRNA and high efficiency reverse transcriptase, such as Moloney murine leukemia virus.
2. Use of bone marrow volumes for reverse transcription results in ______ assay sensitivity.
a) Increased
b) Decreased
c) Fluctuating
d) Positive
View Answer
Explanation: When bone marrow volumes are used for reverse transcription it could lead to decrease in assay sensitivity, because possibilities are there to miss the transcripts at very low levels.
3. RT-PCR is sensitive for BCR transcript detection at very low levels.
a) True
b) False
View Answer
Explanation: Non-quantitative nested RT-PCR is sensitive for BCR/ABL transcript detection at very low levels (10−6), it can be used to verify undetectable real-time Q-PCR results, if desired.
4. Which of these is not a significant factor for optimal real-time Q-PCR?
a) Careful assay design to maximize amplification of pseudogenes
b) Minimal contaminating DNA
c) Amplification of short amplicons
d) Exclusion of polymorphisms in primer- or probe-binding sites
View Answer
Explanation: The most significant factors to ensure optimal real-time Q-PCR are careful assay design to minimize amplification of pseudogenes, minimal contaminating DNA, amplification of short amplicons, exclusion of polymorphisms in primer- or probe-binding sites, and use of proofreading Taq polymerase.
5. How many sequential biochemical activities take place in Retroviral RT?
a) 2
b) 3
c) 4
d) 5
View Answer
Explanation: A sequential biochemical activity that takes place in Retroviral RT has three steps and they are ribonuclease H DNA polymerase activity, RNA-dependent DNA polymerase activity and DNA-dependent DNA polymerase activity.
Sanfoundry Global Education & Learning Series – Agricultural Biotechnology
To practice all areas of Agricultural Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.
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