This set of Agricultural Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Genetic Engineering for Nitrogen Fixation”.
1. In which year did R.A. Dixon transfer nif-genes of K. pneumoniae into E. coli?
a) 1970
b) 1972
c) 1974
d) 1976
View Answer
Explanation: Klebsiella pneumoniae is a non symbiotic nitrogen fixing bacterium. R.A. Dixon and J.R. Postgate transferred nif-genes of K. pneumoniae into E.coli by conjugation in 1972.
2. Nod-genes can increase host range.
a) True
b) False
View Answer
Explanation: Cloned nod-genes of Rhizobium leguminosarum that nodulate pea have been transferred to R. phaseolin. The transformed R. Phaseoli can now nodulate pea plants also.
3. ___________ developed transgenic Arabidopsis thaliana?
a) R.A. Dixon
b) J.R. Postgate
c) Chris Somerville
d) Dereuddre
View Answer
Explanation: Chris Somerville and coworkers have evolved a transgenic Arabidopsis thaliana to polyhydroxy butyrate (PHB). It is a natural and totally biodegradable plastic material. From this plastic producing sugar beet and potatoes can also be produced in future.
4. Pharmaceutical and chemical industries were first to exploit the gene engineering technology.
a) True
b) False
View Answer
Explanation: A futuristic application is envisaged in the field named ‘bioelectronics’ or ‘bionics’. The potential of biological material to serve as extra-sensitive conductors, sensors and support matrices for semi-conductor molecules is already being explored.
5. Which of the following techniques is commonly used in genetic engineering for enhancing nitrogen fixation in plants?
a) CRISPR-Cas9
b) Polymerase Chain Reaction (PCR)
c) RNA interference (RNAi)
d) Southern blotting
View Answer
Explanation: CRISPR-Cas9 is a powerful tool used in genetic engineering to precisely modify DNA sequences. It has been employed to enhance nitrogen fixation in plants by introducing genes responsible for nitrogen-fixing enzymes or by modifying existing genes to improve nitrogen fixation efficiency.
Sanfoundry Global Education & Learning Series – Agricultural Biotechnology
To practice all areas of Agricultural Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.
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