This set of Fermentation Technology Multiple Choice Questions & Answers (MCQs) focuses on “Bioreactor – Vessel Design”.
1. What includes in the biosafety level 1?
a) Respiration protection is required
b) Full body suit
c) Biological safety cabinet
d) Laboratory coat, gloves
View Answer
Explanation: Biosafety level 1 includes personal protective equipment like laboratory coat, gloves, safety glasses. Biosafety level 1 includes standard microbiological practices with facilities like a wash basin.
2. What includes in the biosafety level 2?
a) Respiration protection is required
b) Full body suit
c) Biological safety cabinet
d) Laboratory coat, gloves
View Answer
Explanation: Biosafety level 2 includes a better biological safety cabinet. In addition, it also includes Biosafety level 1 lab coats, gloves, etc. BSL 2 practices include standard operating procedures for decontamination, sharp precautions, limited access and biohazard warning plus BSL 1.
3. The respiratory protection is a part of ______________
a) BSL 1
b) BSL 2
c) BSL 3
d) BSL 4
View Answer
Explanation: The respiratory protection is a part of Biosafety level 3. In addition to it, it also includes the safety equipment of BSL 2. It includes practices like decontamination of laboratory clothing prior to laundering, controlled access plus BSL 2.
4. The use of full body suit is the safety equipment of ______________
a) BSL 1
b) BSL 2
c) BSL 3
d) BSL 4
View Answer
Explanation: The use of full body suit is the safety equipment of Biosafety level 4. It should also include positive pressure with personal air supply plus the equipment of BSL 3. The experiments should be performed in a well-isolated area or a separate building with effluent decontamination and double-ended autoclave.
5. Who of the following is used to produce a cooling effect on the vessel?
a) Baffles
b) Sparger
c) Impeller
d) Stirrer glands
View Answer
Explanation: The baffles are used to produce a cooling effect on the vessel. The cooling coils are connected to the baffles which provide cooling when the heat is generated in the system. It also increases aeration and agitation efficiency.
6. What is the minimum volume of vessel required to produce 1 kg of antibody by batch fermentation?
a) 7 L
b) 500 L
c) 350 L
d) 200 L
View Answer
Explanation: The minimum volume of vessel required to produce 1 kg of antibody by batch fermentation is 500 L. It is used in the production of IgG antibodies from mice. 7 L is required to carry out fed-batch fermentation and 350 L vessel volume is required for continuous fermentation.
7. How much amount of the medium is required for the production of 1 kg antibody in continuous fermentation?
a) 7,000 L
b) 10,000 L
c) 20,000 L
d) 5,000 L
View Answer
Explanation: 7,000 L of the medium is required for the production of 1 kg antibody in continuous fermentation per year. 20,000 L of the medium is required for batch fermentation and 7,000 L is required for the fed-batch fermentation.
8. The heat exchangers are used to _______________
a) Maintain high temperature
b) Maintain low temperature
c) Maintain low pressure
d) Maintain low pressure
View Answer
Explanation: The low or ambient temperature is maintained by internal heat exchangers fitted to the head plate through which cold and hot water are introduced. The jacketed vessel can also be used for this purpose.
9. The ________ is a vessel that contains all the parts and conditions necessary for the growth of desired microorganisms.
a) Impeller
b) Sparger
c) Bioreactor
d) Baffles
View Answer
Explanation: The bioreactor is a vessel that contains all the parts and conditions necessary for the growth of desired microorganisms. It is a vessel that carries out all the fermentation reactions for the production of the desired product.
10. The jacketed vessel is more stable than the non-jacketed vessel.
a) True
b) False
View Answer
Explanation: The jacketed vessel is more stable than the non-jacketed vessel as the jacketed vessel is designed such that it controls the temperature of its contents by using a heating or cooling jacket in the vessel. However, jacketed vessels are much costlier than non-jacketed vessels.
11. Mammalian cell culture processes are of shorter duration.
a) True
b) False
View Answer
Explanation: Mammalian cell culture processes are of longer duration due to the slow growing nature of cells. In fed-batch culture, it may run from 7 to 14 days and in batch fermentation, it lasts for a few days.
12. Which of the following can be used as a quick method of getting liquid into a vessel?
a) Triple inlet
b) Double inlet
c) Septa
d) Single inlet
View Answer
Explanation: Septa can be used as a quick method for getting liquid into the vessel. It consists of a rubber plug housed within a fitting that goes through the head plate which is used in providing liquid into a vessel.
13. Which of the following is not a method for the introduction of fluid in the vessel?
a) Double inlet
b) Triple inlet
c) Single inlet
d) Septa
View Answer
Explanation: The double inlet is not used for the introduction of fluid or liquid into the vessel. The methods used are Single inlet, double inlet, and the Septa. The important advantage of these devices is that they save a lot of space.
14. The solenoid valve _________________
a) Contains the flow tube and the float
b) Is controlled by a signal from an external controller
c) The most expensive way of controlling gas flow
d) Consists of the rotor and housing
View Answer
Explanation: The solenoid valve is controlled by a signal from an external controller operating to control temperature, pH, etc. It can be installed on the outlet side of the rotameter. Whereas a rotameter consists of the flow tube and the float. The mass flow controller is the most expensive way of controlling the gas flow. A peristaltic pump consists of the rotor and housing.
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