This set of Agricultural Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Storage of DNA and Handling of Enzymes”.
1. Hemophilis influenzae helps in conversion of large DNAs into defined fragments.
a) True
b) False
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Explanation: The isolation of the first enzymes was a result of an interesting discovery by Ham Smith and his coworkers that Hemophilis influenzae extracts contained activities that cut large DNAs into defined fragments.
2. In 1970, the existing dogma in molecular biology was that genetic information transfer occurred from RNA to DNA.
a) True
b) False
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Explanation: The proof that RNA to DNA information transfer did occur rested on the discovery and characterization of reverse transcriptase by Temin and Baltimore. Reverse transcriptase allows us to generate DNA copies of mRNA subsequent to cloning.
3. __________ allows us to generate cDNA from mRNA.
a) Polymerase chain reaction
b) Reverse transcriptase
c) Nuclease
d) Molecular scalpels
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Explanation: The generation of cDNA, containing direct protein coding information is the usual step in cloning of eukaryotic genes. Reverse transcriptase allows us to generate DNA copies of mRNA subsequent to cloning.
4. Which among the following is the simplest molecular biology technique?
a) DNA fingerprinting
b) PCR
c) Host controlled modification
d) Cutting of vectors
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Explanation: The most revolutionary and in many aspects the most simplistic molecular biological technical development is PCR. PCR is straightforward application of DNA polymerase to permit the test tube binomial amplification of specific DNA sequences.
5. Which among the following is used in cheese production?
a) Papain
b) Pepsin
c) Renin
d) Urokinase
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Explanation: Papain is an enzyme derived from papaya plant and added to animal feeds as a met tenderizer. Renin is used in cheese production. Pepsin is an animal protease used in the manufacturing industry.
6. Which among the following is used in cancer chemotherapy?
a) Urokinase
b) Asperaginase
b) Renin
d) Papain
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Explanation: Urokinase is used as an anti-thrombotic agent in treatment of pulmonary embolism. Pepsin is an animal protease used in the manufacturing industry. Asperaginase is used in cancer chemotherapy.
7. Which among the following enzymes convert glucose to gluconic acid?
a) Glucose isomerase
b) Glucose oxidase
c) Tyrosine hydroxylase
d) Urokinase
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Explanation: Glucose isomerase converts glucose to fructose. Glucose oxidase converts glucose to gluconic acid which is an industrial chemical and is also used extensively in diagnostic test for measuring blood sugar.
8. Which among the following enzymes help against Parkinson’s disease?
a) Tyrosine hydroxylase
b) Urokinase
c) Glucose oxidase
d) Asperaginase
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Explanation: Tyrosine hydroxylase converts tyrosine into DOPA which is specific against Parkinson’s disease. Urokinase is used as an anti-thrombotic agent in treatment of pulmonary embolism.
9. What is the preferred pH for DNA storage in TE buffer?
a) 7
b) 7.5
c) 8
d) 8.5
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Explanation: DNA can be stored in TE buffer (pH 8.0) or in sterile distilled water at -20˚C. Frequent freezing and thawing of DNA should be avoided to prevent breaks and nicks.
10. What is the time limit for storage of enzymes in ice bucket?
a) 1-2 minutes
b) 2-3 minutes
c) 3-4 minutes
d) 4-5 minutes
View Answer
Explanation: Enzymes should never be stored in their original packing in the freezer at -20˚C and should never be placed on the counter top except in an ice bucket for short 1-2 minute intervals when pipetting is to be carried out.
Sanfoundry Global Education & Learning Series – Agricultural Biotechnology
To practice all areas of Agricultural Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.
