This set of Agricultural Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Gene Cloning Vectors”.
1. Plasmids are not found in eukaryotes.
a) True
b) False
View Answer
Explanation: Plasmids are extrachromosomal double-stranded circular DNA molecules that can be found in various bacteria and some eukaryotes. In their original form, the size of plasmids ranges between 1 and 200 kbp (kilo-base pairs).
2. Plasmids often contain _______ encoding enzymes.
a) Mitochondria
b) Ribosomes
c) Genes
d) Lysosomes
View Answer
Explanation: Plasmids often contain genes encoding enzymes that confer a certain selective advantage to the host cell. Such selective advantages include resistance to certain antibiotics.
3. Elements of certain restriction-modification systems are encoded in plasmid DNA.
a) True
b) False
View Answer
Explanation: The selective advantages include resistance to certain antibiotics. In other cases, the conferred advantage can be the synthesis of antibiotics or various toxins. Elements of certain restriction-modification systems are also encoded in plasmid DNA.
4. Which is the most commonly used host cell?
a) Archaea
b) Streptococcus Bacterium
c) E. coli
d) Streptomyces soil bacterium
View Answer
Explanation: The most commonly applied host cell in recombinant DNA techniques is the bacterium Escherichia coli (E. coli). Therefore, in the rest of this chapter we will focus on plasmids that occur and can be maintained in E. coli.
5. Which of these is possible in cloning vectors themselves?
a) Transcription of gene
b) Translation of gene
c) Storing of genetic sequence
d) Production of gene
View Answer
Explanation: Cloning vectors are only useful for storing a genetic sequence. By themselves, they are incapable of allowing for transcription and translation of the gene into a functional protein product.
Sanfoundry Global Education & Learning Series – Agricultural Biotechnology
To practice all areas of Agricultural Biotechnology, here is complete set of 1000+ Multiple Choice Questions and Answers.
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