This set of Agricultural Biotechnology Multiple Choice Questions & Answers (MCQs) focuses on “Alkaline Phosphate and Terminal Deoxynucleotidyl Transferase”.
1. What is the molecular weight of alkaline phosphate?
a) 11,000
b) 12,000
c) 13,000
d) 14,000
View Answer
Explanation: Alkaline phosphate catalyses the removal of the 5’-terminal phosphate residues from the nucleic acid. This enzyme is a dimeric glycoprotein with a molecular weight of 14,000.
2. Alkaline phosphate consists of identical subunits.
a) True
b) False
View Answer
Explanation: The molecular weight of alkaline phosphate with two identical or similar subunits is of 69,000 each. It is a zinc containing enzyme with four atoms of Zn2+ per molecule.
3. Which of the following is not an application of alkaline phosphate?
a) Mapping
b) Finger printing studies
c) 3’-end labelling of DNA fragments
d) Prevention of Recirculating linearized cloning vectors
View Answer
Explanation: The application of alkaline phosphate includes prevention of linearized cloning vectors from recircularizing by dephosphorylation with alkaline phosphate.
4. _______ is used to end label nucleotides with 32P.
a) Alkaline phosphate
b) Polynucleotide kinase
c) Terminal deoxynucleotidyl transferase
d) S1 nuclease
View Answer
Explanation: Polynucleotide kinase catalyses the transfer of the terminal phosphate group of ATP to the 5’-hydroxylated terminal of DNA or RNA. This enzyme is frequently used to end-label nucleic acids with 32P.
5. What is the molecular weight of TDT enzyme?
a) 16000
b) 18000
c) 25000
d) 32000
View Answer
Explanation: TDT stands for terminal deoxynucleotidyl transferase. It catalyses the repetitive addition of mononucleotide units from a deoxynucleoside triphosphate to the terminal 3’-hydroxyl group of a DNA molecule.
6. TDT is used to add homopolymer tails to DNA fragments.
a) True
b) False
View Answer
Explanation: A unique technique called homopolymer tailing can be used and sticky ends can be built up on blunt-ended molecules.
Sanfoundry Global Education & Learning Series – Agricultural Biotechnology
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