This set of Biochemical Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Active Immobilisation of Cells”.
1. Which of these is an active technique of immobilization?
a) Entrapment
b) Biofilm formation
c) Growth of cell on solid surface
d) Polymerization
View Answer
Explanation: Entrapment, binding are the active techniques of immobilization. Biofilm formation, growth of cell on solid surface is the techniques of passive immobilization.
2. Technique of immobilization can be used to control broth rheology and cell morphology.
a) True
b) False
View Answer
Explanation: Immobilization technique can be used to control rheology and cell morphology. It is also widely used for the detection of concentration of nutrients, metabolites and etc.
3. Which of this is an example of non-specific cross linking?
a) Collagen
b) Al-alginates
c) PAAm
d) Epoxy resins
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Explanation: Collagen crosses links non-specifically to immobilize a catalyst by entrapment process. The network is formed by precipitation.
4. Epoxy resins are used to form a network to immobilize catalysts. Which of these processes result in the network formation by Epoxy resins?
a) Precipitation
b) Ion-exchange gelation
c) Polycondensation
d) Polymerization
View Answer
Explanation: Polycondensation is the process which results in the network formation to immobilize catalyst. The covalent hetero-polymer molecules cross link with each other.
5. Which of these polymer networks can be formed by precipitation method?
a) Al-alginates
b) PAAm
c) PMAAm
d) Polystyrene
View Answer
Explanation: Polystyrene forms a network like structure which results in the formation a polymer which can trap catalyst. The binding process occurs non-specifically.
6. What kind of cross-inking occurs PAAm?
a) Non-specific
b) Ionic
c) Covalent-homopolymer
d) Covalent-heteropolymer
View Answer
Explanation: Network of PAAm is formed by polymerization to immobilize cells. Covalent homopolymer cross-linking is seen.
7. Which of these is an important chemical parameter used for polymer networks of cell entrapment?
a) Geometry
b) Hydrophobic
c) Compressibility
d) Strength
View Answer
Explanation: The important parameters considered to design a polymer network for cell entrapment can be mechanical and chemical. Hydrophobic, Hydrophilic are some of the chemical parameters.
8. Enzymes cannot be entrapped in spun fibers.
a) True
b) False
View Answer
Explanation: Entrapment is a technique used for immobilizing enzymes. Enzymes can be entrapped in spun fibers, in porous hollow fibre, within insoluble gel matrix and within a microcapsule.
9. Which of these is an organic synthetic carrier which supports the immobilized enzyme?
a) Porous Glass
b) Dextran
c) Agarose
d) Polystyrene
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Explanation: Organic synthetic carriers have high mechanical stability and chemical stability. Examples of organic synthetic carriers are polystyrene, Acrylic polymers and etc.
10. Enzyme immobilization can enhance stability and can be re-used. Hence it’s __________
a) Cost effective
b) Does not contaminate the products
c) Degrade
d) Deactivate
View Answer
Explanation: Immobilization can enhance the stability and can be re-used. Hence it is cost effective. Other advantages of Enzyme immobilization are
- It does not contaminate the products.
- Immobilization protects from degradation and deactivation.
Sanfoundry Global Education & Learning Series – Biochemical Engineering
To practice all areas of Biochemical Engineering, here is complete set of 1000+ Multiple Choice Questions and Answers.
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