This set of Biochemical Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Fed Batch Operation & Perfusion System”.
1. Which of these supports the initial cell culture in fed batch operation?
a) Feed medium
b) Base medium
c) Culture medium
d) High product concentration
View Answer
Explanation: In fed batch operation base medium supports the initial cell culture. While a feed medium is used to prevent nutrient depletion.
2. Which of this parameter is affected by the concentration of nutrients in fed-batch culture?
a) pH
b) Cell density
c) Mutation
d) Yield
View Answer
Explanation: Conc. of nutrients directly affects the productivity. Conc. of nutrients affects the yield or productivity of the metabolites.
3. Which of these compounds cannot shorten the lag-time?
a) Methanol
b) Ethanol
c) Uric acid
d) Acetic acid
View Answer
Explanation: Nutrients like methanol, ethanol can reduce the growth of microorganisms. The addition of these nutrients in a proper manner can reduce the lag time and cell inhibition.
4. What is the cell density in a fed batch bioreactor?
a) Low
b) High
c) Equal to the amount of nutrients
d) 0
View Answer
Explanation: The cell density is maintained high in a batch operation reactor. This is mainly done to avoid dilution of the bioreactor feed.
5. How do the cells grow at ideal condition?
a) Steady state
b) Slow growth
c) Exponential growth
d) Higher than steady state
View Answer
Explanation: The cells grow exponentially at the ideal condition. The feed rate of growth limiting substrate is increased in proportion to the growth to maintain the specific growth rate.
6. What is the specific growth rate of cells during batch operation?
a) Constant
b) Increasing
c) Decreasing
d) Increases and then decreases
View Answer
Explanation: The specific growth rate is constant. The feed rate of growth limiting substrate is increased in proportion to the growth to maintain the specific growth rate.
7. What is the molar mass of a component input into a batch bioreactor?
a) V0
b) V
c) V1
d) 0
View Answer
Explanation: The molar mass of input and output streams in fed-batch reactor is 0. The rate of accumulation is equal to the (-) rate of disappearance.
8. Which of this bioreactor used in modern biotech industries to increase the cell concentration when compared to batch/fed batch?
a) Perfusion bioreactor
b) Trickle bed bioreactor
c) Air lift bioreactor
d) Chemostat
View Answer
Explanation: Perfusion bioreactor is used in modern biotech industries to increase the cell concentration and productivity. This technique was discovered in the last 1980s and early 1990s.
9. What is the number of cells developed using perfusion bioreactor earlier?
a) 5×108\(\frac{cells}{ml}\)
b) 5×106\(\frac{cells}{ml}\)
c) 5×104\(\frac{cells}{ml}\)
d) 5×105\(\frac{cells}{ml}\)
View Answer
Explanation: Earlier 5×106 \(\frac{cells}{ml}\) has been developing perusing perfusion bioreactors were developed in mid 1990s. Now the technology has been advanced to form more cells up to 15×104 \(\frac{cells}{ml}\).
10. What is the number of cells developed using perfusion bioreactor in the present?
a) 5×106\(\frac{cells}{ml}\)
b) 8×105\(\frac{cells}{ml}\)
c) 15×106\(\frac{cells}{ml}\)
d) 20×105\(\frac{cells}{ml}\)
View Answer
Explanation: Earlier 5×106 \(\frac{cells}{ml}\) were developed in mid 1990s. Now the technology has been advanced to form more than 15×106\(\frac{cells}{ml}\).
Sanfoundry Global Education & Learning Series – Biochemical Engineering
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