Here are Biochemical Engineering MCQs (Chapterwise).
1. Which of the following is not a primary objective of biochemical engineering?
a) Maximization of waste generation
b) Minimization of production costs
c) Maximization of enzyme activity
d) Optimization of bioprocesses
View Answer
Explanation: Biochemical engineering aims to minimize waste generation while optimizing bioprocesses, maximizing enzyme activity, and minimizing production costs.
2. Which component is added in cell culture media for instant growth?
a) Macronutrients
b) Trace elements
c) Hormones
d) Micronutrients
View Answer
Explanation: Cells derived from organisms require hormones for instant growth in laboratories. The other mentioned options are importantly required for growth throughout the period of culture.
3. Which biologically important peptide can be used as an artificial sweetener?
a) Vasopressin
b) Aspartame
c) Gramicidin
d) Oxytocin
View Answer
Explanation: It is tripeptide. It is widely used in industries as an artificial sweetener.
4. Which of these techniques are used for the characterization of proteins?
a) Electrophoresis
b) Dialysis
c) Chromatography
d) Salting out
View Answer
Explanation: Electrophoresis is a technique of separation of proteins based on the migration of charged proteins under the influence of an electric field. It is also a protein purification technique.
5. Which of these methods is not a continuous enzyme assay?
a) Calorimetric
b) Spectrophotometric
c) Light scattering
d) Chromatographic
View Answer
Explanation: Chromatographic assays measure product formation by separating the reaction mixture into its components. Whereas, the other measures study a particular feature of the mixture for assay, like color, heat and etc.
6. Which enzyme production is increased when the cII protein levels are high?
a) RNA polymerase II
b) RNA polymerase I
c) Integrase
d) DNA dependent RNA polymerase
View Answer
Explanation: The transcription of int gene increases when the cII protein levels are high. This increases the production of the enzyme integrase.
7. Which of these organisms contain lipopolysaccharides in their outer membrane?
a) Staphylococcus aureus
b) Escherichia coli
c) Bacillus cereus
d) Hay bacillus
View Answer
Explanation: Lipopolysaccharides are seen in the outer membrane of gram negative bacteria. All other examples are of gram positive bacteria.
8. What is the primary function of triglycerides?
a) Store fats
b) Hormone production
c) Structural integrity
d) Digestion and absorption
View Answer
Explanation: Triglycerides are formed by esterification of three fatty acid molecules on one glycerol molecule. Their primary function is to store fats for purpose like insulation and absorption etc.
9. Which nucleoside represents a Cytosine base added to the nucleic acid RNA?
a) Deoxycytidylate
b) Cytidylate
c) Deoxycytidine
d) Cytidine
View Answer
Explanation: Cytidine and deoxycystidine are the nucleosides representing the nucleic acid RNA and DNA respectively. Cytidylate and deoxycytidylate are the nucleotides representing the nucleic acid RNA and DNA respectively.
10. Which micronutrient is required to increase the crop yield?
a) K
b) Li
c) Zn
d) Ca
View Answer
Explanation: Zinc deficiency was overcome by the addition of Zinc Fertilizers. These fertilizers helped in the increase of crop yield, balanced the nutrition levels by providing zinc at a cost effective manner.
11. What is the use of Cell counting technique?
a) To determine health of the cells
b) To determine cell variability
c) To determine growth rate
d) All of the above mentioned
View Answer
Explanation: Cell counting techniques are used to quantify cells. The quantification allows to determine the complete activity of the cell.
12. Which of the following is a limitation of batch bioprocessing?
a) Difficulty in controlling conditions
b) High initial investment
c) Slow growth of microorganisms
d) Low product yield
View Answer
Explanation: One of the limitations of batch bioprocessing is the difficulty in controlling conditions such as nutrient availability, pH, and oxygen concentration throughout the entire process.
13. Which of these parameters are kept constant in an ideal chemostat?
a) pH, cell density, concentration of products
b) Concentration of products
c) Cell density
d) pH
View Answer
Explanation: In an ideal chemostat all the parameters are kept constant like pH, concentration of the products, cell density, concentration of the nutrients and etc. Especially the specific growth rate is kept constant.
14. What is the first successful genetically engineered product?
a) Somatotropin
b) Insulin
c) Growth hormone
d) Tobacco
View Answer
Explanation: Insulin is first genetically engineered drug. It is used to treat diabetes.
15. Which was the first host cell species used to discover transduction?
a) Escherichia coli
b) Streptococcus
c) Salmonella
d) Staphylococcus
View Answer
Explanation: Transduction was discovered in 1952 in Salmonella. Norton Zinder and Joshua Lederberg are the scientists who discovered this technique of gene transfer.
16. Which of these is a disease caused by TEs?
a) Sickle cell anemia
b) Turner’s syndrome
c) Down’s syndrome
d) Hemophilia A
View Answer
Explanation: Hemophilia A is a disease caused by Transposons. The other diseases are Hemophilia B, Porphyria and etc.
17. What is the cell density in a fed batch bioreactor?
a) Equal to the amount of nutrients
b) Low
c) High
d) 0
View Answer
Explanation: The cell density is maintained high in a batch operation reactor. This is mainly done to avoid dilution of the bioreactor feed.
18. Which of these is an active technique of immobilization?
a) Biofilm formation
b) Entrapment
c) Polymerization
d) Growth of cell on solid surface
View Answer
Explanation: Entrapment, binding are the active techniques of immobilization. Biofilm formation, growth of cell on solid surface is the techniques of passive immobilization.
Chapterwise Multiple Choice Questions on Biochemical Engineering
- Overview of Biological Basics
- How Cells Grow
- How Cellular Information is Altered
- Modifying Batch and Continuous Reactors
- Immobilized Cell Systems
1. MCQ on Overview of Biological Basics
The section contains multiple choice questions and answers on biological basics, amino acids and proteins, enzymes, viruses, carbohydrates, lipids, fats, and steroids, nucleic acids, and micronutrients and macronutrients.
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2. How Cells Grow
The section covers questions and answers on quantifying cell concentration, cells’ growth patterns, and kinetics in batch culture, as well as the ideal chemostat.
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3. How Cellular Information is Altered
The section explores how cellular information is altered through MCQs covering mutation, genetic recombination, transformation, transduction, episomes and conjugation, and transposons.
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4. Modifying Batch and Continuous Reactors
The section contains multiple choice questions and answers on fed batch operation and perfusion system.
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5. Immobilized Cell Systems
The section covers questions and answers on active immobilisation of cells.
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Wish you the best in your endeavor to learn and master Biochemical Engineering!
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