This set of Biochemical Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Episomes and Conjugation”.
1. Episome is also known as_________
a) Vector
b) Plasmid
c) Chromosomal DNA
d) Chromosomal RNA
View Answer
Explanation: Episome is also called Plasmid. It is small circular DNA which is seen separate from the Chromosomal DNA.
2. Plasmid is seen in Eukaryotes.
a) True
b) False
View Answer
Explanation: Plasmid is small circular double stranded DNA. It is seen in bacteria mostly. Sometimes it is also seen in archea and Eukaryotes.
3. Which of these is seen as an additional feature in plasmids?
a) Origin of replication
b) Multiple cloning sites
c) Restriction sites
d) Antibiotic resistance gene
View Answer
Explanation: Plasmids contain antibiotic resistance gene. This gene allows plasmid to become a selectable marker by providing it an ability to grow in media containing antibiotics.
4. What size of DNA can be cloned using plasmids?
a) 10kbp
b) 12kbp
c) 15kbp
d) 18kbp
View Answer
Explanation: Typically DNA of size 15kbp can be cloned using plasmids. The longer length DNA is cloned with the help of cosmids, artificial chromosomes of yeast and bacteria.
5. In which was the term episomes introduced?
a) 1956
b) 1958
c) 1960
d) 1962
View Answer
Explanation: The term episomes were introduced in the year 1958 by Francois Jacob and Elie Wollman. It is the extra-chromosomal genetic material which can integrate with the chromosome or replicate by itself.
6. What is the size of Plasmid used for genetic engineering of yeast?
a) 0.5 µm
b) 1 µm
c) 1.5 µm
d) 2 µm
View Answer
Explanation: Yeast naturally has variety of plasmids. The size of the plasmid used in genetic engineering of yeast is 2 µm.
7. Which technique can be used to determine the conformation of Plasmid DNA?
a) Chromatography
b) Electrophoresis
c) Lowry’s test
d) DNS test
View Answer
Explanation: Plasmid DNA can be separated according to its conformation by using the technique Electrophoresis. The separation occurs in the order Nicked open-circular DNA, Relaxed circular DNA, Linear DNA, Supercoiled DNA, Supercoiled denatured DNA.
8. Which of these applications cannot record the DNA sequence of Plasmid vectors?
a) ApE
b) LabGenius
c) pDraw32
d) MathLab
View Answer
Explanation: MathLab cannot be used to record the DNA sequence of Plasmid vectors. The software’s which can be used are ApE, LabGenius, pDraw32, Clone Manager, Genome Compiler and etc.
Sanfoundry Global Education & Learning Series – Biochemical Engineering
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