This set of Biochemical Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Biological Basics – Amino Acids and Proteins”.
1. What is the purpose of Glutathione?
a) Prevent damage to RBC
b) Hormones which cause rise in blood pressure
c) Artificial sweetener
d) Antibiotic
View Answer
Explanation: Glutathione is a biologically important peptide. It is a tripeptide which prevents oxidative damage to RBC.
2. Light produced by fireflies is the result of the protein ___________
a) Coelenterazine
b) Oxyluciferin
c) Aequorin
d) Luciferin
View Answer
Explanation: Light produced by firefly is due to the oxidative reaction involving luciferin and ATP. The reaction is catalyzed by the enzyme luciferase.
3. Which technique helps in the separation of proteins based of affinity?
a) Chromatography
b) Dialysis
c) Salting out
d) Centrifugation
View Answer
Explanation: Chromatography is a separation techniques based on size, affinity, charge and etc. Affinity chromatography is used for the separation of proteins based on their binding affinity.
4. The absolute configuration of amino acids is specified by the D, L system.
a) True
b) False
View Answer
Explanation: Special nomenclature is used to specify absolute configuration based on the four substitutes of asymmetric carbon atom. Hence absolute configuration is specified by the D,L system.
5. N-Cα and Cα-C can rotate at a bond angle ψ and ϕ respectively.
a) True
b) False
View Answer
Explanation: The peptide bond is partially double bond which keeps the six atom peptide bond in a rigid planar configuration. So N-Cα and Cα-C can rotate at a bond angle ϕ and ψ respectively.
6. Which amino acid contains sulphur in it?
a) Cysteine
b) Aspargine
c) Serine
d) Histidine
View Answer
Explanation: The molecular formula are:-
Cysteine-C3H7NO2S, Aspargine-C4H8N2O3, Serine-C3H7NO3, Histadine-C6H9N3O2
7. What is the isoelectric point of Glycine?
a) 5.79
b) 5.97
c) 5.079
d) 5.709
View Answer
Explanation: The Isoelectric point is calculated using the equation:
pI=\(\frac{1}{2}\)(pk1+pk2)=\(\frac{1}{2}\)(2.34+9.60)=5.97
8. Which biologically important peptide can be used as an artificial sweetener?
a) Gramicidin
b) Oxytocin
c) Vasopressin
d) Aspartame
View Answer
Explanation: It is tripeptide. It is widely used in industries as an artificial sweetener.
9. What does this structure represent?
a) D-threonine
b) L-threonine
c) D-allothreonine
d) L-allothreonine
View Answer
Explanation: The structures of the other options are:-
10. How is a protein structure stabilized?
a) Electrostatic forces
b) Weak interaction forces
c) Multiple weak interaction forces
d) Metallic bond forces
View Answer
Explanation: Protein structure is stabilized by multiple weak interaction forces like hydrogen bonds, hydrophobic bonds and etc.
11. Which is a nine amino acid peptide?
a) Gramicidin
b) Vasopressin
c) Glutathione
d) Aspartame
View Answer
Explanation: Vasopressin is a nine amino acid peptide. It is a hormone used to increase blood pressure by constricting peripheral blood vessels.
12. Identify the structure of protein based on the explanation given below:
“This structure provides a stable arrangement of amino acids residues giving rise to recurring structural patterns.”
a) Primary Structure
b) Secondary structure
c) Tertiary structure
d) Quaternary structure
View Answer
Explanation: This structure provides a stable arrangement of amino acids residues giving rise to recurring structural patterns is called secondary structure. It consists of alpha and beta sheets.
Sanfoundry Global Education & Learning Series – Biochemical Engineering
To practice all areas of Biochemical Engineering, here is complete set of 1000+ Multiple Choice Questions and Answers.
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