Biophysics MCQ (Multiple Choice Questions)

Here are Biophysics MCQs (Chapterwise).

1. What is the primary focus of biophysics?
a) Study of plant physiology
b) Examination of animal behavior
c) Investigation of biological processes using physical principles
d) Exploration of geological formations in living organisms
View Answer

Answer: c
Explanation: Biophysics is about using the principles of physics to understand how living things work. It helps us study and analyze different processes that happen in living organisms.

2. In the context of biophysics, what does the acronym DNA stand for?
a) Deoxyribonucleic Acid
b) Diatomic Nitrogen Atom
c) Dynamic Nucleotide Assembly
d) Dichloroethane Nitrate Amide
View Answer

Answer: a
Explanation: DNA, or Deoxyribonucleic Acid, is a special molecule that carries important information about how living things grow and function.

3. What is the minimum required centrifugal force to separate DNA from a mixture of cell components?
a) 60000 g
b) 40000 g
c) 20000 g
d) 10000 g
View Answer

Answer: d
Explanation: DNA is the most commonly available part compared to other cell organelles present in the cell. So just to separate DNA one can centrifuge the cell suspension at low centrifugation that is 10000 rpm.

4. Which technique is commonly used in biophysics to study the structure of biological macromolecules?
a) Polymerase chain displacement reaction (PCDR)
b) High-performance liquid chromatography (HPLC)
c) Nuclear magnetic resonance (NMR) spectroscopy
d) Polymerase chain reaction (PCR)
View Answer

Answer: c
Explanation: In biophysics, scientists often use a technique called Nuclear Magnetic Resonance (NMR) spectroscopy to closely examine the structure of big molecules in living things.

5. Which technique uses Scattering to reveal an unknown protein structure?
a) X ray crystallography
b) NMR
c) Fluorescence spectrophotometry
d) CD spectrophotometry
View Answer

Answer: a
Explanation: In X-ray crystallography, we first make a protein crystal and followed by its purification, we place it in front of laser light and observe the scattering of light. Observing the scattering pattern, the molecular structure is predicted. NMR does not use scattering. CD and Fluorescence are used to quantify proteins not used to determine the structure.

6. What is the primary function of a biophysicist in the study of living organisms?
a) Conducting archaeological research
b) Analyzing weather patterns
c) Investigating historical linguistics
d) Applying physical principles to understand biological phenomena
View Answer

Answer: d
Explanation: Biophysicists play a key role in understanding living organisms by using the rules of physics to study how different living things work and interact. They help unravel the mysteries of biology using the tools of physics.

7. What do you infer from the word diffusion?
a) It requires ATP
b) It’s moving from higher concentration to lower concentration
c) It is not getting transported at all
d) It’s moving from lower concentration to higher concentration
View Answer

Answer: b
Explanation: The process where one molecule moves from its higher concentration to its lower concentration is called a diffusion process. Now the correct answer mentions that the movement of the drug is from a higher to a lower concentration.

8. Paper chromatography separates molecules based on which property?
a) Polarity
b) Molecular weight
c) Shape
d) Viscosity
View Answer

Answer: a
Explanation: Paper chromatography separates molecules based on its polarity. The concept is the more it’s attracted to the stationary phase the less it is travel in the paper chromatography.

9. What type of adsorption chromatography uses the liquid as a mobile phase?
a) Gas-Liquid Chromatography
b) Gas – Gas Chromatography
c) Gas-Solid Chromatography
d) Liquid-Solid Chromatography
View Answer

Answer: d
Explanation: Liquid-Solid Chromatography uses the liquid as a mobile phase. The adsorption of molecules happens on a solid surface. In a Liquid-Solid Chromatography, liquid works as a mobile phase.

10. What is the basic principle of HPLC?
a) It is a gas-gas partition chromatographic technique
b) It is a liquid-solid partition chromatographic technique
c) It is a liquid-liquid partition chromatographic technique
d) It is a gas-solid partition chromatographic technique
View Answer

Answer: c
Explanation: HPLC is a technique to separate two liquids. Therefore, It is a liquid-liquid partition chromatographic technique.

11. Which of the following material is used in the anion exchange column?
a) Diethyl-aminoethyl groups (DEAE)
b) Bio-Rex 70
c) SP-Sepharose
d) DEAE-Sephadex
View Answer

Answer: a
Explanation: Diethyl-aminoethyl groups (DEAE) is used in the anion exchange column. The component materials of the following column are -CH2CH2N(C2H5)2 which is a weak base. As the material is positive in nature so it is used as an ion exchange column.

12. Which of the following beads can be used to separate proteins with 50kd, 80kd and 30kd?
a) Sephadex G- 50
b) Sephadex G-100
c) Sephadex G- 10
d) Sephadex G- 25
View Answer

Answer: b
Explanation: The standard elusion range of Sephadex G-100 is 4-150KD. Therefore, it is suitable for the separation of the above-mentioned proteins. Whereas, the rest of the beads are used to separate smaller sized proteins.

13. What is the chemical property of the matrix used in an affinity chromatography?
a) Basic
b) Acidic
c) Inert
d) Ionic
View Answer

Answer: c
Explanation: The matrix should be inert so that it does not interact with the eluting proteins. Another reason is that the matrix should not chemically interact with the bound ligands so that the ligand remains unchanged.

14. What is the number of amino acids present in a Hemoglobin molecule, if each α chain and β chain contains 141 and 146 amino acids respectively in it?
a) 574
b) 282
c) 292
d) 428
View Answer

Answer: a
Explanation: Hemoglobin molecule is made up of 4 polypeptide chains, 2 α chain and 2 β chain.
It is given that no. of amino acids in α chain = 141 and β chain = 146.
Thus, the total number of amino acids in Hemoglobin molecule =
2 * (no. of amino acids in α chain) + 2 * (no. of amino acids in β chain) = 2 * (141) + 2 * (146) = 574.

15. Which of the following bonds does not help in stabilizing Tertiary structure of protein?
a) Disulphide bond
b) Ionic bond
c) Peptide Bond
d) Hydrogen Bond
View Answer

Answer: c
Explanation: The tertiary structure of a protein is stabilized by mainly Ionic, Disulphide and Hydrogen bond whereas, peptide bond helps in linking various amino acids to form proteins.

16. What is the significance of the sodium-potassium pump in cellular biophysics?
a) Monitoring seismic activity
b) Analyzing ancient pottery
c) Controlling atmospheric pressure
d) Regulating ion concentrations within cells
View Answer

Answer: d
Explanation: The sodium-potassium pump plays a crucial role in maintaining the balance of ions within cells, essential for cell function and communication.

17. What is used to detect hydrocarbon spots in TLC place with silica gel?
a) KMnO4
b) Alkaline Tetrazolium
c) UV light exposure
d) Ninhydrin test
View Answer

Answer: c
Explanation: UV light exposure is used to detect hydrocarbon spot in TLC place with silica gel. The hydrocarbons absorb short wavelength UV light but the solvent doesn’t, so hydrocarbons can be visible to UV light.

Chapterwise Multiple Choice Questions on Biophysics

Biophysics MCQ

Our MCQs focus on all topics of the Biophysics subject, covering all topics. This will help you to prepare for exams, contests, online tests, quizzes, viva-voce, interviews, and certifications. You can practice these MCQs chapter by chapter starting from the 1st chapter or you can jump to any chapter of your choice.
  1. Physico-Chemical Techniques to Study Biomolecules
  2. Diffusion
  3. Separation Techniques
  4. Macromolecular Structure

1. MCQ on Physico-Chemical Techniques to Study Biomolecules

The section contains multiple choice questions and answers on centrifugation and scattering.

  • Centrifugation
  • Centrifugation – Set 2
  • Scattering
  • 2. Diffusion

    The section covers questions and answers on diffusion.

  • Diffusion
  • Diffusion – Set 2
  • 3. Separation Techniques

    The section covers various separation techniques, including Thin Layer Chromatography, Paper Chromatography, Adsorption Chromatography, Partition Chromatography, Ion Exchange Chromatography, Molecular Exclusion Chromatography, and Affinity Chromatography.

  • Separation Techniques
  • Thin Layer Chromatography
  • Paper Chromatography
  • Paper Chromatography – Set 2
  • Adsorption Chromatography
  • Partition Chromatography
  • Partition Chromatography – Set 2
  • Ion Exchange Chromatography
  • Ion Exchange Chromatography – Set 2
  • Molecular Exclusion Chromatography
  • Molecular Exclusion Chromatography – Set 2
  • Affinity Chromatography
  • 4. Macromolecular Structure

    The section contains multiple choice questions and answers on protein structure.

  • Protein Structure
  • If you would like to learn "Biophysics" thoroughly, you should attempt to work on the complete set of 1000+ MCQs - multiple choice questions and answers mentioned above. It will immensely help anyone trying to crack an exam or an interview.

    Wish you the best in your endeavor to learn and master Biophysics!

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