Pre-requisite / Skillset for Vector Biology and Gene Manipulation Internship
1. Intern must have adequate knowledge on various cloning and expression vectors.
2. Require knowledge of vectors in genetic engineering experiments.
3. Adequate knowledge on various strategies of gene cloning.
4. Must be aware of importance genetic engineering.
5. Must be familiar with safety and ethical considerations while manipulating and engineering biological systems.
6. Overview on the principles of heredity and gene manipulation.
7. Must be familiar with the concepts like gene manipulation that can be applied to engineer simple and complex life forms and safety considerations associated with genetic engineering.
Vector Biology and Gene Manipulation Internship Test
Intern has to take Vector Biology internship test in which they will be asked 50 multiple-choice questions. They have to score grade A or A* on this test to be eligible for the internship. Here’s a sample set of questions on the Vector Biology topic.
1. Protection by covalent attachment of proteins in a plasmid, is a method for?
(A) increasing the conjugative abilities
(B) protection of linear DNA
(C) increasing the copy number
(D) increasing cloning efficiency
Explanation: Not all plasmids exist as circular molecules, linear plasmids have been found in some species and to protect them, methods are: repeated sequences ending in terminal DNA hairpin loop or the ends are protected by covalent attachment of a protein.
2. What is the role of ethidium bromide in isopycnic centrifugation of cleared lysates?
(A) Denatures genomic DNA
(B) Lysis the membrane
(C) Intercalates and unwinds
(D) Fluoresces plasmid DNA
Explanation: EtBr binds by intercalating between the DNA basepairs and in doing so causes the plasmid DNA to unwind. Thus covalent circles can be separated from linear chromosomal DNA.
3. What are sticky ends?
(A) Ends of M13 vector
(B) 12 nucleotide stretch in lambda phage
(C) The replicated product phage DNA
(D) Ends on two sides of origin
Explanation: At either end of the lambda DNA molecule is a short 12 nucleotide stretch in which the DNA is single stranded. The two single strands are complementary and base pair with each other to form a circular completely double stranded molecule.
4. Which of the following is a positive regulatory gene on the lambda phage genome?
Explanation: Except for the two positive regulatory genes N and Q, all functionally related genes are clustered together on the phage lambda genome map.
5. The restriction endonuclease AluI is isolated from which microbe?
(A) Proteus Vulgaris
(B) Staphylococcus Aureus
(C) Arthrobacter Luteus
(D) Haemophilus Influenzae
Explanation: AluI restriction endonuclease derived from Arthrobacter Luteus, cleaves a DNA molecule at nucleotide long site- AGCT. There are other endonucleases that cleave at bigger sites.
Practice the full set of Vector Biology Multiple Choice Questions before applying for the internship.
How to apply for the Vector Biology Internship
1. Read the Sanfoundry Internship FAQs
2. Take the Vector Biology Internship Test
3. Submit the Internship Application Form
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