Tissue Engineering Questions and Answers – Bioreactors for Liver Tissue Engineering

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This set of Tissue Engineering Multiple Choice Questions & Answers (MCQs) focuses on “Bioreactors for Liver Tissue Engineering”.

1. Yin et al. developed a cultivation system to differentiate mouse embryoid bodies (EBs) into the hepatocytes using a _________ bioreactor.
a) spinner
b) photo
c) electronic
d) piezoelectric
View Answer

Answer: a
Explanation: Yin et al., 2007, built up a development framework to separate mouse embryoid bodies (EBs) into the hepatocytes utilizing a spinner bioreactor. They found that this framework could be utilized as an enormous scale hepatic separation framework.
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2. Primary hepatocytes and stem cells cultured on Rotating Wall Vessel reactors.
a) TRUE
b) FALSE
View Answer

Answer: a
Explanation: Essential hepatocytes and stem cells refined on RWV reactors were additionally used to design Bio-artificial Liver (BAL), since these frameworks may limit disturbance and shear pressure and expand mass exchange. For instance, Ishikawa et al. discovered that the hepatocytes exhibited a bile-channel like or vessel-like structure following 10 days of culture utilizing an RWV bioreactor framework.

3. Why are Perfusion bioreactors preferred over Rotating Wall Vessel Bioreactors for Bio-artificial Liver production?
a) Controlled environment
b) The capacity is more
c) Efficiency is more
d) The material of perfusion bioreactors is inert
View Answer

Answer: a
Explanation: Cell development isn’t constantly uniform in RWV frameworks, and cells might be harmed because of crashes between the platforms and the reactor dividers. Perfusion frameworks permit a progressively controlled condition, consistent presentation of gas and supplements, and end of waste items. This design permits the perfusion of the medium or the patient’s blood/plasma through a lattice loaded up with liver cells.
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4. Which of the following are suitable for prolonged in vitro studies?
a) Dielectric-fiber bioreactors
b) Hollow Fiber bioreactors
c) Optical Fiber bioreactors
d) Rotating Wall Vessel Bioreactors
View Answer

Answer: b
Explanation: Albeit various sorts of perfusion bioreactors have improved the liver cell work, the Hollow fiber bioreactors (HFB) framework is the most normally utilized setup in TE applications. There are a few reports on the replication of liver tissue capacities utilizing HFB frameworks both in vitro and in vivo models. On account of medication and toxicology testing, these frameworks have generally been utilized in light of the fact that they are appropriate for drawn out in vitro investigations.

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Manish Bhojasia - Founder & CTO at Sanfoundry
Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He is Linux Kernel Developer & SAN Architect and is passionate about competency developments in these areas. He lives in Bangalore and delivers focused training sessions to IT professionals in Linux Kernel, Linux Debugging, Linux Device Drivers, Linux Networking, Linux Storage, Advanced C Programming, SAN Storage Technologies, SCSI Internals & Storage Protocols such as iSCSI & Fiber Channel. Stay connected with him @ LinkedIn | Youtube | Instagram | Facebook | Twitter