This set of Fermentation Technology Multiple Choice Questions & Answers (MCQs) focuses on “Fermentation Laboratory – Supporting Facilities”.
1. The pilot-scale fermenters require _________________
a) Single phase supply
b) Three-phase supply
c) Two-phase supply
d) Five-phase supply
View Answer
Explanation: The pilot-scale fermenters require three phase supply for each fermenter or control unit, steam generator, and tubular bowl centrifuge. The three-phase supply is a common method to transfer power and is used to power heavy loads.
2. The cooling water is used to ____________
a) Prevent overgrowth
b) Increase protease activity
c) Enhance growth
d) Cell culturing
View Answer
Explanation: The cooling water has the ability to cool the culture and this is used upon cell harvesting, both to prevent the overgrowth, and also reduce the activity of the protease. The minimum temperature of the cooling water in the laboratory is approximately 15°C.
3. The pipework in SIP fermenters much be made up of grade ____________
a) 316 stainless steel
b) 317 stainless steel
c) 318 stainless steel
d) 319 stainless steel
View Answer
Explanation: The pipework in SIP fermenters must be made up of grade 316 stainless steel. Otherwise, it is possible for rust to enter the jacket and the fermenter might get damaged potentially. The other grades must be avoided at any costs.
4. Which of the following is not the use of compressed air?
a) Provide oxygen to the broth
b) Activate valves
c) Vessel emptying
d) Prevent Rusting
View Answer
Explanation: The compressed air has several functions in the fermenter. It is used to provide oxygen to the broth and is also used to activate pneumatic valves. It is also used to increase the rate of emptying the vessel.
5. Which of the following regulates gas flow electronically?
a) Rotameter
b) MFC
c) Centrifuge
d) Incubator
View Answer
Explanation: Thermal mass flow controllers are devices that regulate the gas flow electronically. It determines the gas flow by the rate at which heat is absorbed from a heat source.
6. What is the use of ‘blanket’ of inert nitrogen gas?
a) Promotes aerobic condition development
b) Prevents aerobic condition development
c) Promotes anaerobic condition development
d) Prevents anaerobic condition development
View Answer
Explanation: In anaerobic cultures, the use of ‘blanket’ of inert nitrogen gas is to promote the development of anaerobic conditions in the liquid phase by the exclusion of gas. This blanket is highly pure, oxygen-free nitrogen.
7. Off-gas analysis can be done by ______________
a) UV Spectrophotometer
b) Mass spectrophotometer
c) UV Transilluminator
d) Incubator
View Answer
Explanation: Off-gas analysis can be done with the help of mass spectrophotometer. It is highly accurate but a little bit expensive. Some of the cheaper and low accurate gas analyzers are TanDem, EGAS-L, or FerMac analyzers.
8. Which of the following is used to measure the generated heat?
a) Calorimeter
b) UV Spectrophotometer
c) UV Transilluminator
d) Mass spectroscopy
View Answer
Explanation: Basically all the processes result in heat production or absorption. Microcalorimeter is used in the measurement of the generated heat as a function of time. It is useful for research on metabolic activities of a cellular system and other biological conditions.
9. The calorimetry is not used for __________________
a) Measurement of heat generation
b) Recording growth
c) Recording substrate production
d) Off-gas analysis
View Answer
Explanation: The calorimetry is used for the measurement of heat generation as a function of time. It can also be used for recording growth, product formation, substrate production. It cannot be used for off-gas analysis of microbiological cultures.
10. Which of the following is used in measuring the absorbance?
a) UV-visible spectrophotometer
b) UV transilluminator
c) Incubator
d) Calorimeter
View Answer
Explanation: UV-visible spectrophotometer is used in the measurement of absorbance or optical density of a biological sample such as protein, DNA, RNA, etc. It uses the light in UV and visible ranges. Transilluminator is used for viewing DNA or RNA bands after electrophoresis. The incubator is used to provide optimum conditions for the growth of microbes whereas calorimeter is used in the measurement of heat generation.
11. The distilled water is used to ___________
a) Prepare defined media
b) Gain weight
c) Prepare food
d) Be healthy
View Answer
Explanation: The distilled water is commonly used to prepare defined media as it does not contain any ions or minerals. It cannot be used for the preparation of food and be healthy because our body needs minerals and ions which are not present in distilled water.
12. Which of the following is a software responsible for regulating the growth parameters?
a) Oracle
b) Java
c) SCADA
d) C++
View Answer
Explanation: SCADA is the software responsible for regulating the growth parameters. It is user-friendly. Java, Oracle, Turbo C++ are the software of programming languages.
13. Who used the calorimetry to measure flux in yeast?
a) Hans Westerhoff
b) Jacob
c) Monad
d) Antoce
View Answer
Explanation: Hans Westerhoff along with his colleagues used the microcalorimetry to measure the total fluxes in growing yeast cells. Some other techniques like fluorimetry showed that oscillations in the concentrations of glycolytic intermediates in yeast cells depend on the growth phase in which they are harvested.
14. The TanDem analyzer has a range of 0-30% for measuring oxygen.
a) True
b) False
View Answer
Explanation: The standard TanDem analyzer is suitable for measuring oxygen between the ranges of 0-30%. The analyzer can measure carbon-dioxide in the range of 0-5%. It has an accuracy of 0.02% and a resolution of 0.01%.
15. Mass spectrophotometer separates ions by their charge-to-mass ratio.
a) True
b) False
View Answer
Explanation: The mass spectrophotometer separates ions based on their mass-to-charge ratios. It ionizes the chemical species and then separates or sorts ions into a spectrum. It is used in the measurement of charged particles or ions.
Sanfoundry Global Education & Learning Series – Fermentation Technology.
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