This set of Class 12 Biology Chapter 11 Multiple Choice Questions & Answers (MCQs) focuses on “Tools of Recombinant DNA Technology: Restriction Enzymes”.
1. From which organism was the first restriction enzyme isolated?
a) Escherichia coli
b) Salmonella typhimurium
c) Bacillus cereus
d) Staphylococcus aureus
View Answer
Explanation: The restriction enzymes are molecular scissors that cleave the DNA at specific recognition sites. They were first isolated from Escherichia coli in the year 1963.
2. In genetic engineering, restriction enzymes cleave the DNA at a specific site known as _____
a) restriction
b) recognition
c) promoter
d) sense
View Answer
Explanation: Restriction enzymes cleave the DNA at a specific sequence known as recognition sequence. These sequences are highly specific for each enzyme.
3. Restriction enzymes are also known as _________
a) ligase
b) polymerase
c) telomerase
d) restriction endonucleases
View Answer
Explanation: Restriction enzymes are also known as restriction endonucleases or restrictase. They are chemical knives (scissors) used in genetic engineering or recombinant DNA technology.
4. _____ was the first restriction endonuclease was isolated and characterized.
a) EcoRI
b) BamHI
c) Hind II
d) Sma I
View Answer
Explanation: Hind II was the first restriction endonuclease to be isolated and characterized. It cuts the DNA by recognizing a specific sequence of six base pairs. It was isolated from the bacteria called Haemophilus influenzae.
5. Restriction enzymes belong to a larger class of enzymes called ______
a) proteins
b) proenzyme
c) nucleases
d) isozymes
View Answer
Explanation: Restriction enzymes belong to a larger class of enzymes called nucleases. There are two types of nucleases. They are: exonucleases and endonucleases.
6. The _______ in DNA are base pair sequences that are the same when read forward or backward from a central axis of symmetry.
a) nucleases
b) palindrome
c) termination sequences
d) amino acids
View Answer
Explanation: Palindromes are a group of letters that form the same words when read forward (left to right) or backward (right to left). For example: NAYAN, MALAYALAM, etc.
7. Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______
a) polymerase chain reaction
b) gene amplification
c) blotting
d) gel electrophoresis
View Answer
Explanation: DNA is a charged molecule. When fragmented, it can be separated by the technique called gel electrophoresis. It is a technique in which the molecules are separated based on the charge present on that molecule.
Sanfoundry Global Education & Learning Series – Biology – Class 12.
To practice all chapters and topics of class 12 Biology, here is complete set of 1000+ Multiple Choice Questions and Answers.
- Get Free Certificate of Merit in Biology - Class 12
- Participate in Biology - Class 12 Certification Contest
- Become a Top Ranker in Biology - Class 12
- Take Biology - Class 12 Tests
- Chapterwise Practice Tests: Chapter 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- Chapterwise Mock Tests: Chapter 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- Practice Class 12 - Physics MCQs
- Buy Class 12 - Biology Books
- Practice Class 12 - Chemistry MCQs
- Practice Class 12 - Mathematics MCQs
- Practice Class 11 - Biology MCQs
