Biology Questions and Answers – Tools of Recombinant DNA Technology: Restriction Enzymes

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This set of Biology Multiple Choice Questions & Answers (MCQs) focuses on “Tools of Recombinant DNA Technology: Restriction Enzymes”.

1. From which organism was the first restriction enzyme isolated?
a) Escherichia coli
b) Salmonella typhimurium
c) Bacillus cereus
d) Staphylococcus aureus
View Answer

Answer: a
Explanation: The restriction enzymes are molecular scissors that cleave the DNA at specific recognition sites. They were first isolated from Escherichia coli in the year 1963.
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2. In genetic engineering, restriction enzymes cleave the DNA at a specific site known as _____
a) restriction
b) recognition
c) promoter
d) sense
View Answer

Answer: b
Explanation: Restriction enzymes cleave the DNA at a specific sequence known as recognition sequence. These sequences are highly specific for each enzyme.

3. Restriction enzymes are also known as _________
a) ligase
b) polymerase
c) telomerase
d) restriction endonucleases
View Answer

Answer: d
Explanation: Restriction enzymes are also known as restriction endonucleases or restrictase. They are chemical knives (scissors) used in genetic engineering or recombinant DNA technology.
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4. _____ was the first restriction endonuclease was isolated and characterized.
a) EcoRI
b) BamHI
c) Hind II
d) Sma I
View Answer

Answer: c
Explanation: Hind II was the first restriction endonuclease to be isolated and characterized. It cuts the DNA by recognizing a specific sequence of six base pairs. It was isolated from the bacteria called Haemophilus influenzae.

5. Restriction enzymes belong to a larger class of enzymes called ______
a) proteins
b) proenzyme
c) nucleases
d) isozymes
View Answer

Answer: c
Explanation: Restriction enzymes belong to a larger class of enzymes called nucleases. There are two types of nucleases. They are: exonucleases and endonucleases.
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6. The _______ in DNA are base pair sequences that are the same when read forward or backward from a central axis of symmetry.
a) nucleases
b) palindrome
c) termination sequences
d) amino acids
View Answer

Answer: b
Explanation: Palindromes are a group of letters that form the same words when read forward (left to right) or backward (right to left). For example: NAYAN, MALAYALAM, etc.

7. Fragments of DNA formed after treatment with endonucleases are separated by the technique of _______
a) polymerase chain reaction
b) gene amplification
c) blotting
d) gel electrophoresis
View Answer

Answer: d
Explanation: DNA is a charged molecule. When fragmented, it can be separated by the technique called gel electrophoresis. It is a technique in which the molecules are separated based on the charge present on that molecule.
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