Analytical Instrumentation Questions and Answers – High Pressure Liquid Chromatography

This set of Analytical Instrumentation Multiple Choice Questions & Answers (MCQs) focuses on “High Pressure Liquid Chromatography”.

1. Which of the following is not true about High pressure liquid chromatography (HPLC)?
a) It requires high pressure for the separation of the specious
b) There is no need to vaporise the samples
c) It is performed in columns
d) It has high sensitivity
View Answer

Answer: b
Explanation: In High pressure liquid chromatography (HPLC), samples need to be vaporised. It has high sensitivity.

2. High pressure liquid chromatography can be performed only in columns.
a) True
b) False
View Answer

Answer: a
Explanation: High pressure liquid chromatography can be performed only in columns. This is because the application of high pressure in the open bed will not be effective.

3. Which of the following is not an advantage of Syringe type pumps used in High pressure liquid chromatography?
a) Independent of viscosity
b) Pulse-less flow
c) High pressure capability
d) Unlimited solvent capacity
View Answer

Answer: d
Explanation: The limitation of Syringe type pump is that it has a limited solvent capacity and is inconvenient when solvents are to be changed.
advertisement
advertisement

4. Which of the following is not true about solvent programming which is done in high performance liquid chromatography?
a) It provides unequal bandwidths
b) It provides fast overall separation
c) It provides maximum resolution
d) It provides maximum sensitivity
View Answer

Answer: a
Explanation: Solvent programming is done to provide equal bandwidths. It provides maximum sensitivity for every solute in the sample.

5. Which of the following pulse damper takes up some amount of the pulsation energy which is released to provide smooth pressure without pulsations?
a) Flexible bellows or compressible gas passed through tee columns
b) Flexible inert diaphragm
c) Electronic pulse damper
d) Electrical pulse damper
View Answer

Answer: a
Explanation: Flexible bellows or compressible gas passed through tee columns take up some of the pulsation energy which is released to provide smooth pressure without pulsations. Its main purpose is to avoid pulses.

6. Which of the following is not a characteristic of the syringe pump used in high pressure liquid chromatography?
a) Pressure capability is high
b) Maintenance is frequent
c) Limited reservoir capability
d) Slight change of flow rate when extremely high pressure compresses the solvent
View Answer

Answer: b
Explanation: In syringe pumps, maintenance is infrequent. However, it has high pressure capability.

7. Syringe pumps used in High pressure liquid chromatography are most suitable for which of the following columns?
a) Capillary columns
b) Guard columns
c) Short-fast columns
d) Small bore columns
View Answer

Answer: d
Explanation: Syringe pumps used in High pressure liquid chromatography are most suitable for small bore columns. It is the most commonly used piston type pump.
advertisement

8. Gravity feed method for solvent delivery is not used with narrow bore columns packed with fine mesh particles.
a) True
b) False
View Answer

Answer: a
Explanation: Gravity feed method for solvent delivery is not used with narrow bore columns packed with fine mesh particles. This is because they cannot deliver solvent at high pressure.

9. Which of the following cannot be done to reduce ripple in High pressure liquid chromatography?
a) Using bellows
b) Using restrictors
c) Using long nylon tube between pump and column
d) Avoiding the use of the solvent pump
View Answer

Answer: d
Explanation: Solvent pumps have to be used to reduce pulses in a solvent flow. When there is a pulsed flow, the efficiency of column decreases.
advertisement

10. Which of the following is not true about Hydraulic capacitance flow control system used in HPLC?
a) It can be used only for liquids with low viscosity
b) It is irrespective of solvent compressibility
c) It maintains a constant flow
d) It smoothens high pressure pump pulsations
View Answer

Answer: a
Explanation: Hydraulic capacitance flow control system used in HPLC is irrespective of the solvent viscosity. It is also irrespective of the solvent compressibility.

Sanfoundry Global Education & Learning Series – Analytical Instrumentation.

To practice all areas of Analytical Instrumentation, here is complete set of 1000+ Multiple Choice Questions and Answers.

If you find a mistake in question / option / answer, kindly take a screenshot and email to [email protected]

advertisement
advertisement
Subscribe to our Newsletters (Subject-wise). Participate in the Sanfoundry Certification contest to get free Certificate of Merit. Join our social networks below and stay updated with latest contests, videos, internships and jobs!

Youtube | Telegram | LinkedIn | Instagram | Facebook | Twitter | Pinterest
Manish Bhojasia - Founder & CTO at Sanfoundry
Manish Bhojasia, a technology veteran with 20+ years @ Cisco & Wipro, is Founder and CTO at Sanfoundry. He lives in Bangalore, and focuses on development of Linux Kernel, SAN Technologies, Advanced C, Data Structures & Alogrithms. Stay connected with him at LinkedIn.

Subscribe to his free Masterclasses at Youtube & discussions at Telegram SanfoundryClasses.