Analytical Instrumentation Questions and Answers – Column Efficiency and Column Resolution

This set of Analytical Instrumentation Questions and Answers for Freshers focuses on “Column Efficiency and Column Resolution”.

1. Which of the following is the distance that the solute moves while undergoing one partition?
a) Retention distance
b) Distribution constant
c) Plate height
d) Column packing length

Explanation: Plate height is the distance that the solute moves while undergoing one partition. It is expressed in length.

2. Which of the following conditions will cause the efficiency of the column to increase?
a) Plate number becomes greater, plate height becomes smaller
b) Plate number becomes smaller, plate height becomes smaller
c) Plate number becomes greater, plate height becomes larger
d) Plate number becomes greater, plate height becomes larger

Explanation: The efficiency of the column increases when the plate number becomes greater and the plate height becomes smaller. Column efficiency greatly depends on plate height and plate number.

3. Which of the following is the expression for Eddy diffusion in the column, if ‘γ’ represents obstruction factor, ‘dp’ represents particle diameter, ‘DM’ represents Solute diffusion co-efficient and ‘λ’ represents function for packing uniformity?
a) λ dM
b) 2 γ DM
c) DM γ
d) λ/dM

Explanation: Eddy diffusion is given as, A = λ dp. It is also called multipath term.

4. Which of the following is the expression for longitudinal diffusion in the column, if ‘γ’ represents obstruction factor, ‘dM’ represents particle diameter, ‘DM’ represents Solute diffusion co-efficient and ‘λ’ represents function for packing uniformity?
a) λ dM
b) 2 γ DM
c) DM γ
d) λ/dM

Explanation: Longitudinal diffusion is given as, B = 2 γ DM.It is also called axial diffusion.

5. In gas-liquid chromatography, when films are used in the interior of the capillary column, then what is the value of Eddy diffusion?
a) Greater than 1
b) Less than 1
c) Zero
d) Less than zero

Explanation: In gas-liquid chromatography, when films are used in the interior of the capillary column the value of Eddy diffusion (A) is zero. To minimize A, diameter of packing should be small and uniform.
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6. For an unpacked coated capillary column, the obstruction factor takes which of the following values?
a) 0
b) 0.6
c) 1
d) 1.6

Explanation: For an unpacked coated capillary column, the value of the obstruction factor is 1. For a packed column, the value is 0.6.

7. Base line resolution is achieved when degree of resolution/separation is ______
a) 1
b) 0
c) 0.5
d) 1.5

Explanation: Base line resolution is achieved when degree of resolution/separation is 1.5. The baseline bandwidths of adjacent bands are almost constant.

8. Which of the following equations give the expression for plate number, N when ‘tR’ is the adjusted retention time and ‘Wb’ is the width at the base of the peak which is equal to 4 standard deviations.
a) 16 tR2/Wb
b) 4 tR2/Wb
c) (4tR/Wb)2
d) 4 (tR/Wb)2

Explanation: The expression for plate number, N is given as N = (4tR/Wb)2. It is a dimensionless quantity.

9. It is more difficult to measure the width at half peak height than the base width in the detector output.
a) True
b) False

Explanation: The width at half peak height is easier to measure than the base width. Hence, the plate number is calculated at peak height.

10. The solute, while moving in the column is always in equilibrium with both the stationary phase and the mobile phase.
a) True
b) False

Explanation: The solute, while moving in the column is always in equilibrium with both the stationary phase and the mobile phase. But, the two phases will never be in equilibrium with each other.

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