This set of Engineering Chemistry Multiple Choice Questions & Answers (MCQs) focuses on “Thin Layer Chromatography”.
1. The principle on which thin layer chromatography is based is that the ____________
a) Different compounds are absorbed on an absorbent to different degrees
b) Different compounds are absorbed on an absorbent to same degrees
c) Different compounds are adsorbed on an adsorbent to different degrees
d) Different compounds are absorbed on an absorbent to same degrees
View Answer
Explanation: Thin layer chromatography is based on the fact that different compounds are adsorbed on an adsorbent to different degrees. It is a chromatography technique used to separate non-volatile mixtures.
2. The size of a thin layer of adsorbent is about ____________
a) 0.1 mm
b) 0.2 mm
c) 0.3 mm
d) 0.4 mm
View Answer
Explanation: The size of a thin layer of adsorbent is about 0.2 mm. TLC plates are usually commercially available, with standard particle size ranges to improve reproducibility.
3. The chromaplate or thin layer chromatography plate is made up of ____________
a) Glass
b) Wood
c) Fibre
d) Metal
View Answer
Explanation: The chromaplate or thin layer chromatography plate is made up of glass. TLC plates are usually commercially available, with standard particle size ranges to improve reproducibility. They are prepared by mixing the adsorbent, such as silica gel, with a small amount of inert binder like calcium sulfate (gypsum) and water.
4. The eluant filled in the closed jar is ____________
a) Mixture of gases
b) Mixture of a liquid and a gas
c) Mixture of solids
d) Mixture of liquids
View Answer
Explanation: The eluant filled in the closed jar is liquid or mixture of liquids.
5. Select the incorrect statement from the following options.
a) The spots of colorless compounds are invisible to the eyes
b) These spots can be detected by putting the plate under ultraviolet light
c) These spots can be detected by placing the plate in a covered jar containing iodine crystals
d) None of the mentioned
View Answer
Explanation: All the mentioned options are correct. The spots of colorless compounds are invisible to the eyes, can be detected by putting the plate under ultraviolet light and can be detected by placing the plate in a covered jar containing iodine crystals.
6. The relative adsorption of each component of the mixture is expressed in terms of its ____________
a) Acceleration factor
b) Retardation factor
c) Both acceleration and retardation factor
d) None of the mentioned
View Answer
Explanation: The relative adsorption of each component of the mixture is expressed in terms of its retardation factor (Rf).
7. Select the correct statement from the following.
a) Paper chromatography is a type of partition chromatography
b) A special quality paper is used in paper chromatography
c) Chromatography paper contains water trapped in it, which acts as stationary phase
d) All of the mentioned
View Answer
Explanation: Paper chromatography is a type of partition chromatography. A special quality paper is used in paper chromatography which contains water trapped in it, which acts as a stationary phase.
8. Amino acids detected by spraying the plate with ninhydrin solution is an example of ____________
a) Column chromatography
b) Thin layer chromatography
c) Paper chromatography
d) Liquid chromatography
View Answer
Explanation: Amino acids detected by spraying the plate with a ninhydrin solution is an example of paper chromatography. Paper chromatography is an analytical method that is used to separate colored chemicals or substances.
9. Paper chromatography is based on continuous differential partitioning of components of a mixture between stationary and mobile phases.
a) True
b) False
View Answer
Explanation: Paper chromatography is based on a continuous differential partitioning of components of a mixture between stationary and mobile phases. It is an analytical method that is used to separate colored chemicals or substances.
10. Retardation factor is the ratio of ____________
a) Distance moved by substance from base line to distance moved by the solvent from base line
b) Distance moved by solvent from base line to distance moved by the substance from base line
c) Distance moved by substance from top line to distance moved by the solvent from top line
d) Distance moved by solvent from top line to distance moved by the substance from top line
View Answer
Explanation: Retardation factor is the ratio of the distance moved by substance from base line to distance moved by the solvent from base line.
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