This set of Biochemistry Multiple Choice Questions & Answers (MCQs) focuses on “DNA Recombination”.
1. Which of the following involves remarkable capacity of a short segment of DNA to move from one place to another?
a) DNA transposition
b) DNA replication
c) Translation
d) Transcription
View Answer
Explanation: A short segment of DNA with the remarkable capacity to move from one location in a chromosome to another.
2. Which of the following process occurs between DNA molecules of very similar sequences?
a) Homologous genetic recombination
b) Site specific recombination
c) Non-homologous recombination
d) Replicative recombination
View Answer
Explanation: Non-homologous recombination occurs in regions where no large –scale sequence similarity is apparent.
Site-specific recombination occurs between particular short sequences present on otherwise dissimilar parental molecules. Replicative recombination generates a new copy of the transposable element at a new location of DNA.
3. Which of the following process occurs in regions where no large –scale sequence similarity is apparent?
a) Homologous genetic recombination
b) Site specific recombination
c) Non-homologous recombination
d) Replicative recombination
View Answer
Explanation: Site-specific recombination occurs between particular short sequences present on otherwise dissimilar parental molecules.
Replicative recombination generates a new copy of the transposable element at a new location of DNA.
Homologous genetic recombination occurs between DNA molecules of very similar sequences.
4. Which of the following process generates a new copy of the transposable element at a new location of DNA?
a) Homologous genetic recombination
b) Site specific recombination
c) Non-homologous recombination
d) Replicative recombination
View Answer
Explanation: Homologous genetic recombination occurs between DNA molecules of very similar sequences.
Site-specific recombination occurs between particular short sequences present on otherwise dissimilar parental molecules.
Non-homologous recombination occurs in regions where no large –scale sequence similarity is apparent.
5. Which of the following occurs between particular short sequences present on otherwise dissimilar parental molecules?
a) Homologous genetic recombination
b) Site specific recombination
c) Non-homologous recombination
d) Replicative recombination
View Answer
Explanation: Homologous genetic recombination occurs between DNA molecules of very similar sequences.
Non-homologous recombination occurs in regions where no large –scale sequence similarity is apparent.
Replicative recombination generates a new copy of the transposable element at a new location of DNA.
6. Which of the following promotes branch migration at higher rates than does Rec-A?
a) Rec-B
b) Rec-C
c) Rec-D
d) Ruv-A and Ruv-B
View Answer
Explanation: Ruv-A and Ruv-B proteins form a complex that binds to Holliday intermediates, displays Rec-A protein and promotes branch migration at higher rates than does Rec-A.
7. Which of the following is called a resolvase?
a) Ruv-C
b) Ruv-A
c) Ruv-B
d) Rec-A
View Answer
Explanation: Nucleases that specifically cleave Holliday intermediates are often called resolvases, the Ruv-C protein is one of at least two such nucleases in E. coli.
8. Which of the following does not code for an enzyme having both helicase and nuclease activity?
a) Rec-A
b) Rec-B
c) Rec-C
d) Rec-D
View Answer
Explanation: In E. coli, rec-B, rec-C and rec-D genes encode the Rec-BCD enzyme, which has both helicase and nuclease activities.
9. The sequences of the recombination sites recognized by site-specific recombinases are ____________
a) Partially asymmetric
b) Partially symmetric
c) Symmetric
d) Palindromic
View Answer
Explanation: The sequences of the recombination sites recognized by site-specific recombinases are partially asymmetric (non-palindromic).
10. Which of the following contains only the sequences required for transposition and the genes for proteins that promote the process?
a) Insertion sequences
b) Complex transposons
c) Transposons
d) Chromosomes
View Answer
Explanation: Complex transposons contain one or more genes in addition to those needed for transposition.
Sanfoundry Global Education & Learning Series – Biochemistry.
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