Biochemistry Questions and Answers – Fates of Pyruvate under Anaerobic Conditions: Fermentation

This set of Biochemistry Problems focuses on “Fates of Pyruvate under Anaerobic Conditions: Fermentation”.

1. Which of the following is true about the enzyme producing NADH from a triose phosphate in the glycolytic pathway?
a) It produces 1, 3-biphosphoglycerate and NADH
b) It catalyzes irreversible reaction
c) It uses NAD⁺ and dihydroxyacetone phosphate as substrates
d) It uses FADH₂ and glyceraldehyde 3-phosphate as substrates
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2. Which is the major factor(s) determining whether glucose is oxidized by aerobic or anaerobic glycolysis?
a) Ca⁺²
b) FADH₂
c) NADH and the ATP/ADP ratio
d) Presence of high AMP
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3. When glucose is converted to lactate by anaerobic glycolysis, equivalent number of ATPs derived is?
a) 1
b) 2
c) 3
d) 4
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4. When one molecule of glucose is oxidized to two molecules of lactate during anaerobic glycolysis, which of the following statements is false?
a) Glyceraldehyde 3-P dehydrogenase reaction produces 2 ATP molecules
b) Lactate dehydrogenase reaction produces no ATP
c) Pyruvate kinase reaction produces 2 ATP molecules
d) Phosphofructokinase-1 reaction uses 1 ATP molecule
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5. In the reduction of pyruvate to lactate, which of the following is regenerated?
a) H⁺
b) NADH
c) NAD⁺
d) Na⁺
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6. For its activity, pyruvate decarboxylase requires ______________
a) Mg⁺²
b) Ca⁺²
c) Na⁺
d) H⁺
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7. TPP (thiamine pyrophosphate) is derived from ____________
a) Vitamin A
b) Vitamin B1
c) Vitamin C
d) Vitamin B2
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8. What are the end products in ethanol fermentation?
a) Ethanol and CO₂
b) Ethanol and O₂
c) Ethanol, H₂ and CO₂
d) Ethanol, O₂ and CO₂
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9. Which enzyme is involved in the pathway of ethanol fermentation?
a) Hexokinase
b) Pyruvate decarboxylase
c) Pyruvate dehydrogenase
d) Pyruvate kinase
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10. Which enzyme is involved in the pathway of synthesis of acetyl-coA?
a) Hexokinase
b) Pyruvate decarboxylase
c) Pyruvate dehydrogenase
d) Pyruvate kinase
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